Analytes not found
Analytes not found
Column: | ||||||||||||
Brand Name: | ||||||||||||
Part No: | ||||||||||||
Elution Type: Gradient | ||||||||||||
Mobile Phase: A: 0.1% Formic acid in Water B: 0.1% Formic acid in Acetonitrile | ||||||||||||
Gradient Profile:
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Flow Rate: 0.3 mL/min | ||||||||||||
Temperature: 40°C | ||||||||||||
System: Agilent Technologies Agilent Technologies | ||||||||||||
Detection: LC/MS |
Description: bioZen MagBeads, Streptavidin Coated - 500mg, Ea |
Part Number: |
Sample Notes: |
1 Dispense 25 µL of bead slurry into a 96 well plate |
2 Transfer 96 well plate onto magnetic stand for 30 seconds and discard liquid |
3 Add 500µL PBS Buffer to each plate well that contains beads |
4 Vortex bead slurry using mixer or pipette |
5 Transfer 96 well plate onto magnetic stand for 30 seconds and discard liquid |
6 Add 500µL PBS Buffer to each plate well that contains beads |
7 Vortex bead slurry using mixer or pipette |
8 Transfer 96 well plate onto magnetic stand for 30 seconds and discard liquid |
9 Add 500µL PBS Buffer to each plate well that contains beads |
10 Vortex bead slurry using mixer or pipette |
11 Transfer 96 well plate onto magnetic stand for 30 seconds and discard liquid |
12 Reconstitute beads with PBS buffer to the original slurry volume. |
13 Add 10 µL of biontinylated goat anti-human IgG (0.5mg/mL). |
14 Transfer plate for incubation for one hour at room temperature with a shaking speed of 1200 RPM using a deep well plate thermoshaker. Beads should stay suspended during incubation |
15 Transfer 96 well plate onto magnetic stand for 30 seconds and discard liquid |
16 Add 500µL PBS Buffer to each plate well that contains beads |
17 Shake and mix the bead slurry, using vortex or pipette |
18 Transfer 96 well plate onto magnetic stand for 30 seconds and discard liquid |
19 Add 500µL PBS Buffer to each plate well that contains beads |
20 Shake and mix the bead slurry, using vortex or pipette |
21 Transfer 96 well plate onto magnetic stand for 30 seconds and discard liquid |
22 Add 500µL PBS Buffer to each plate well that contains beads |
23 Shake and mix the bead slurry, using vortex or pipette |
24 Transfer 96 well plate onto magnetic stand for 30 seconds and discard liquid |
25 Reconstitute beads with PBS buffer to the original slurry volume. |
26 Shake and mix slurry of anti-human IgG coated beads well, using vortex or pipette |
27 Transfer 50 µL of bead slurry into each well that contains 25 µL sample. Mix well before transferring |
28 Add cover to plate using polyester plate film |
29 Vortex to mix plate |
30 Transfer plate for incubation for one hour at room temperature with a shaking speed of 1200 RPM using a deep well plate thermoshaker. Beads should stay suspended during incubation |
31 Transfer 96 well plate onto magnetic stand for 30 seconds and discard liquid |
32 Add 200 µL of PBS Buffer to each well that contains beads |
33 Vortex well and place plate on centrifuge for 2 minutes at 400RPM |
34 Transfer 96 well plate onto magnetic stand for 30 seconds and discard liquid |
35 Add 200µL of 10mM Ammonium Bicarbonate to each well that contains beads |
36 Vortex well and place plate on centrifuge for 2 minutes at 400RPM |
37 Transfer 96 well plate onto magnetic stand for 30 seconds and discard liquid |
38 Add 100µL of 0.1% TFA in water to each well and vortex |
39 Add pH paper to check pH value (should be lower than 3) |
40 Add plate cover and shake for 10 minutes at 1200RPM using thermomixer |
41 Transfer plate on centrifuge for 2 minutes at 400RPM |
42 Transfer 96 well plate onto magnetic stand for 5 minutes and collect supernatant |