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Optimized HPLC Method for Assay and Separation of Semaglutide from its Known Impurities/ Related Substances using Aeris Peptide XB-C18
In this application note, precise high-performance liquid chromatography (HPLC) techniques were established for the quantitative analysis of Semaglutide and its eight known related substances, utilizing the Aeris™ Peptide XB-C18 HPLC column. This column is specifically engineered for peptide-based separations. XB-C18 ligands featuring di-isobutyl side chains yield a uniformly bonded stationary phase with increased ligand spacing relative to conventional C18 phases, allowing for more effective interactions of longer (>20 residues) peptides with the stationary phase. Additionally, the core-shell particle morphology minimizes analyte diffusion resulting in narrower peaks, providing outstanding resolution and peak shape.
Optimized HPLC Method for Assay and Separation of Semaglutide from its Known Impurities/ Related Substances using Aeris Peptide XB-C18
In this application note, precise high-performance liquid chromatography (HPLC) techniques were established for the quantitative analysis of Semaglutide and its eight known related substances, utilizing the Aeris™ Peptide XB-C18 HPLC column. This column is specifically engineered for peptide-based separations. XB-C18 ligands featuring di-isobutyl side chains yield a uniformly bonded stationary phase with increased ligand spacing relative to conventional C18 phases, allowing for more effective interactions of longer (>20 residues) peptides with the stationary phase. Additionally, the core-shell particle morphology minimizes analyte diffusion resulting in narrower peaks, providing outstanding resolution and peak shape.
Document Type:
Technical Notes
Target Industries:
Techniques:
Brands:
Separation Modes:
Reversed Phase
Phases:
Optimized HPLC Method for Assay and Separation of Semaglutide from its Known Impurities/ Related Substances using Aeris Peptide XB-C18
In this application note, precise high-performance liquid chromatography (HPLC) techniques were established for the quantitative analysis of Semaglutide and its eight known related substances, utilizing the Aeris™ Peptide XB-C18 HPLC column. This column is specifically engineered for peptide-based separations. XB-C18 ligands featuring di-isobutyl side chains yield a uniformly bonded stationary phase with increased ligand spacing relative to conventional C18 phases, allowing for more effective interactions of longer (>20 residues) peptides with the stationary phase. Additionally, the core-shell particle morphology minimizes analyte diffusion resulting in narrower peaks, providing outstanding resolution and peak shape.
| Target Industries: | Pharmaceutical |
| Techniques: | HPLC Reversed Phase |
| Brands: | Aeris HPLC Column |
| Separation Modes: | Reversed Phase |
| Phases: | Aeris Peptide XB-C18 |
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide.
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide.