Biozen dSEC-1
High-Resolution Aggregate Analysis for Peptide Therapeutics
Biozen dSEC-1 columns are engineered to meet the growing demand for aggregate analysis in peptide therapeutics and mid-sized biologics. Packed with 90 Å small pore silica and featuring inert diol-based surface chemistry, dSEC-1 delivers enhanced resolution for low and high molecular weight peptide aggregates. Its robust design ensures reproducibility and compatibility with advanced workflows like SEC-MS and SEC-MALS.
Robust Performance for Peptide Aggregate Analysis
- High resolution for low/high MW peptide aggregates (1–50 kDa)
- Reproducible, and fast analysis with extended column lifetime
- Improved peak asymmetry by up to 40%
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Aggregate and Monomer Separation in Liraglutide Formulations
- Baseline resolution of monomer and two aggregates achieved in under 10 minutes
- Exceptional reproducibility with retention time RSD ≤ 0.08% and peak area RSD ≤ 0.01%
- Clear detection at low levels with S/N ratios of 13, 75, and 10,917 for aggregates and monomer
Column:
Biozen dSEC-1, 1.6 μm
Dimension:
150 x 4.6 mm
Part No:
00F-4801-E0
Mobile Phase (isocratic):
1X PBS:Isopropanol (70:40 v/v)
Flow Rate:
0.35 mL/min
Injection Volume:
20 µg on column
Temperature:
40 ˚C
LC System:
Waters H-class
Detection:
UV@ 280 nm
High-Resolution SEC for siRNA Sequence Differentiation
- Clear resolution achieved between closely related species differing by just three bases
- Robust SEC performance enables confident ID and quantification of CholTEG-conjugated siRNA
- Ideal for therapeutic oligo workflows requiring high resolution and reproducibility
Sequence Name
Bases
Sequence
MW
19 mer Oligo std
19
TTA GGT CCC AAA AGG AGA C
5845.9
16 mer Oligo std
16
GTG CAT CTT CTG AGG T
4903.2
Analytes (Diluent):
ChoITEG conjugated siRNA (Water)
Column:
Biozen dSEC-1, 3 µm
Dimension:
300 x 4.6 mm
Part No.:
00H-4801-E0
Temperature:
10 ˚C
Flow Rate:
0.10 mL/min
Mobile Phase (isocratic):
10 mM Potassium phosphate (pH 7.0), 200 mM Potassium chloride, 40%, Acetonitrile
Injection Volume:
1 µL
Instrument:
Thermo Vanquish Horizon
Wavelength (nm):
UV @ 260 nm
Separation of Sense/Antisense Strands in CholTEG-Conjugated siRNA
- Excellent resolution of duplex, sense (5900 Da), and antisense (6900 Da) strands achieved
- Supports formulation and stability studies through clear resolution of structurally related species
Sequence Name
Bases
Sequence
MW
Sense (S)
16
N/A - Chl1TEG
5900
Antisense (A)
21
N/A
6900
Analytes (Diluent):
ChoITEG conjugated siRNA (Water)
Column:
Biozen dSEC-1, 3 µm
Dimension:
300 x 4.6 mm
Part No.:
00H-4801-E0
Temperature:
10 ˚C
Flow Rate:
0.10 mL/min
Mobile Phase (isocratic):
10 mM Potassium phosphate (pH 7.0), 200 mM Potassium chloride, 40%, Acetonitrile
Injection Volume:
1 µL
Instrument:
Thermo Vanquish Horizon
Wavelength (nm):
UV @ 260 nm
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Order Biozen dSEC-1
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