With a new titanium BioTi™ biocompatible hardware to minimize priming, four particle platforms for optimal versatility and nine particle chemistries to maximize selectivity and sensitivity, Biozen UHPLC/HPLC columns are seamlessly designed to bring peace of mind to your analysis of biologics through:
Keep your mind at ease knowing that we’ve minimized the need for priming with a new titanium infused biocompatible hardware and frit that doesn’t interfere with protein or peptide integrity!
Through a proprietary thermal processing series of steps, we eliminate micropores and further improve consistency, column efficiency, inertness, ruggedness, and reproducibility.
Using sol-gel processing techniques that incorporate nano structuring technology, a durable, homogeneous porous shell is grown on a solid silica core. This highly optimized process combined with industry leading column packing technology produces highly reproducible columns that generate extremely high efficiencies and sensitivity.
Meticulously controlled monosized particle technology secures incredible particle consistency that leads to improved and reliable efficiency. This innovative non-porous particle serves as the perfect backbone for complex ion-exchange chemistries.
The Biozen dSEC columns are packed with low pore volume silica coupled with a proprietary hydrophilic diol-type bonded surface chemistry that prevents the silica surface from interacting with protein samples.
Digested mAbs or ADCs typically include a large body of com pounds which are crucial to understanding post translation modifications. So we designed two Biozen Peptide columns to offer highly useful and unique retention profiles. Each allows for fast and effective elution windows by utilizing either high efficiency core-shell or thermally modified fully porous particles to gain sharper peaks, better peak capacities, and overall higher sensitivity.
Column:
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Dimensions
150 x 2.1 mm
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Part No.:
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Mobile Phase:
A: 0.1 % Formic Acid in Water
B: 0.1 % Formic Acid in Acetonitrile |
Gradient:
Time (min)
0
0.5
50
55
56
| % B
1
1
50
50
95 |
Flow Rate:
0.3 mL/min |
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Temperature:
40 °C |
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Detection:
QTOF (SCIEX® X500B) |
Column:
Biozen 1.8 µm dSEC-2, 200 Å
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Dimensions
300 x 4.6 mm
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Part No.:
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Mobile Phase:
200 Potassium Phosphate +
250 mM KCl, pH 6.2 |
Part No.:
00H-4787-E0
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Flow Rate:
0.35 mL/min
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Injection Volume:
10 µL
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Temperature:
25 °C
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Detection:
UV @ 280 nm
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Sample:
Various,10 mg/mL
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Column:
Biozen 6 μm WCX
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Dimensions
250 x 4.6 mm
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Part No.:
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Mobile Phase:
A: 20 mM MES (pH 5.6)
B: 20 mM MES + 300 mM NaCl (pH 5.6) |
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Gradient:
Time (min)
0
1
31
31.1
34
35
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% B
15
15
45
100
100
15
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Flow Rate:
1 mL/min
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Temperature:
30 °C
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Detection:
UV @ 280 nm
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Sample:
Trastuzumab
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Column:
Biozen 6 μm WCX
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Dimensions
250 x 4.6 mm
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Part No.:
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Mobile Phase:
A: CX -1 (pH 5.6) pH Gradient Buffer*
B: CX -1 (pH 10.2) pH Gradient Buffer*
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Gradient:
Time (min)
0
1
21
23
24
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% B
0
0
100
100
0
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Flow Rate:
1 mL/min
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Temperature:
30 °C
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Detection:
UV @ 280 nm
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Sample:
Trastuzumab
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* From Thermo Fisher Scientific® Inc.
Column:
Biozen 2.6 µm Glycan
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Dimensions
150 x 2.1 mm
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Part No.:
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Mobile Phase:
A: 100 mM Ammonium Formate, pH 4.5
B: Acetonitrile |
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Gradient:
Time (min)
0
10
24
38.5
38.6
40.6 40.7 48 |
% B
78
74.5 72 55.9 40 40 78 78 |
Flow Rate:
0.5 mL/min
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Temperature:
50 °C
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Detection:
FLD ex/em 285/345 nm
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Sample:
As noted
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When quantitating signature peptides from biological matrices, you need sharp peak shape and sufficient retention of hydrophilic peptides to prevent any signal loss from matrix suppression regions. Both Biozen Peptide columns were developed to deliver excellent selectivity for even closely related peptides. Additionally, they build on this body of valuable characteristics with unique ways of delivering sharper peak shape for basic peptides; Biozen Peptide XB-C18 blocks secondary surface interactions via isobutyl side chains, while the Biozen Peptide PS-C18 contains a positively charged weak base that repels other basic species.
Column:
Biozen 3 µm Peptide PS-C18
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Dimensions
50 x 2.1 mm
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Part No.:
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Mobile Phase:
A: 0.1 % Formic Acid in Water
B: 0.1 % Formic Acid in Acetonitrile |
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Gradient:
Time (min)
0
1
4.5
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%B
3
3
25
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Flow Rate:
0.5 mL/min
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Temperature:
22 °C |
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LC System:
ExionLC™ AD HPLC |
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Detection:
MS/MS
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Detector:
SCIEX QTRAP® 5500
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Flow Rate:
1.85 mL/min
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Sample:
As noted
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Column:
Biozen 3.6 µm Intact XB-C8
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Dimensions
150 x 2.1 mm
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Part No.:
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Mobile Phase:
A: 0.1 % TFA in Water
B: 0.1 % TFA in Acetonitrile
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Gradient:
Time (min)
0
1
13
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% B
20
20
25
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Flow Rate:
0.5 mL/min
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Temperature:
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Detection:
UV @ 280 nm
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Sample:
1. Trastuzumab
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Column:
Biozen 3.6 µm Intact XB-C8
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Dimensions
150 x 2.1 mm
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Part No.:
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Mobile Phase:
A: 0.1 % TFA in Water
B: 0.1 % TFA in Acetonitrile
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Gradient:
Time (min)
0
1
13
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% B
20
20
60
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Flow Rate:
0.5 mL/min
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Temperature:
80 °
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Detection:
UV @ 280 nm
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Sample:
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Column:
Biozen 3.6 µm Intact XB-C8
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Dimensions
150 x 2.1 mm
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Part No.:
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Mobile Phase:
A: 0.1 % TFA in Water
B: 0.1 % TFA in Acetonitrile
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Gradient:
Time (min)
0
1
13
|
% B
20
20
45
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Flow Rate:
0.5 mL/min
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Temperature:
80 °
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Detection:
UV @ 280 nm
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Sample:
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Column:
Biozen 3.6 µm Intact XB-C8
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Dimensions
150 x 2.1 mm
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Part No.:
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Mobile Phase:
A: 0.1 % Formic Acid in Water
B: 0.1 % Formic Acid in Acetonitrile /
Isopropyl alcohol (50:50
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Gradient:
Time (min)
2.5
10
110.1
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% B2
0
65
95
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Flow Rate:
0.3 mL/min
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Temperature:
90 °
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Detection:
QTOF (SCIEX X500B)
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Sample:
Trastuzumab
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With a direct effect on efficacy and safety, conjugation for each ADC must be well understood. The Biozen Intact XB-C8 provides an excellent vehicle for determining drug load distribution and DAR for ADCs. Its large pore size allows intact ADCs to interact with a moderately retentive stationary phase while the core-shell particle supplies increased efficiency to deliver the required resolution between ADC species with differing drug loads.
Oligos can chelate to trace heavy metals in stainless steel column hardware, leading to poor recovery, inconsistent chromatography and problematic carryover. The Biozen Oligo bio-inert hardware provides greater sensitivity as well as improved recovery, demonstrating this column’s optimal utility for oligonucleotide characterization and quantitation.
Impurity profiling and characterization of intact biologic fragments is a challenging undertaking because of the need to identify very small differences between variants. Biozen WidePore C4 columns contain skillfully manufactured large pore core-shell particles that provide narrower, taller peaks in conjunction with higher resolution between the target HC/LC, Fc/Fab, or isoforms and are ideal for large biologics to optimize analysis.
Column:
Biozen 2.6 µm WidePore C4
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Dimensions
100 x 2.1 mm
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Part No.:
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Mobile Phase:
A: 0.1 % Formic Acid in Water
B: 0.1 % Formic Acid in Acetonitrile |
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Gradient:
Time (min)
0
4
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% B
10
90
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Flow Rate:
0.3 mL/min
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Temperature:
80 °
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Detection:
UV @ 280 nm
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Sample:
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Sample | Retention Time (min) |
Width @ 50% |
---|---|---|
Rituximab | 3.580 | 0.0233 |
Infliximab Biosimilar | 3.606 | 0.0272 |
Cetuximab | 3.696 | 0.0270 |