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biozen-peptide-polar-c18

Biozen Peptide Polar C18

Enhanced Selectivity for Polar Peptides—Without Sacrificing Retention

Analyzing complex peptide mixtures, especially those rich in polar sequences, demands column chemistries that balance selectivity with broad retention.

Biozen Peptide Polar-C18 is a reversed-phase column engineered to enhance retention of polar peptides while preserving critical non-polar interactions for comprehensive peptide mapping and quantitation.

Built on Phenomenex’s thermally modified particle technology, it offers greater peak capacity, sharper peak shapes, and reliable reproducibility—ideal for challenging digests like mAbs, ADCs, and other biologics.

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Overview

Enhanced retention, sensitivity and resolution

EICs of polar peptides EEQYNSTYR with G0F and G2F Glycan modifications at N5 and VTITCSASSR on a Biozen Peptide Polar C18 (Blue), XB-C18 (Green) and PS (Purple) columns and respective Resolution (Rs) values.

  • Biozen 1.6 µm Peptide Polar C18
  • Biozen Peptide 2.6 µm XB-C18
  • Biozen 3 µm Peptide PS C18
Peptide Type
Peptide
RT
Peak Width
Resolution
Peptide Polar C18
EEQYN(G2F)STYR
19.95
0.16
Column5
EEQYN(GF0)STYR
20.15
Column4
Column5
VTITCSASSR
22.39
0.22
10.57
Peptide XB-C18
EEQYN(G2F)STYR
16.45
0.21
EEQYN(GF0)STY
16.54
0.22
0.44
VTITCSASSR
18.5
0.22
8.88
Peptide PS C18
EEQYN(G2F)STYR
17.24
0.36
EEQYN(GF0)STYR
17.38
0.28
0.44
VTITCSASSR
18.45
0.22
4.28