Biozen Peptide Polar C18
Enhanced Selectivity for Polar Peptides—Without Sacrificing Retention
Analyzing complex peptide mixtures, especially those rich in polar sequences, demands column chemistries that balance selectivity with broad retention.
Biozen Peptide Polar-C18 is a reversed-phase column engineered to enhance retention of polar peptides while preserving critical non-polar interactions for comprehensive peptide mapping and quantitation.
Built on Phenomenex’s thermally modified particle technology, it offers greater peak capacity, sharper peak shapes, and reliable reproducibility—ideal for challenging digests like mAbs, ADCs, and other biologics.
Separation Mode Reversed Phase |
Pore Size 100 |
Recommended Use Enhanced selectivity/retention for polar analytes in a nano flow format |
Enhanced retention, sensitivity and resolution
EICs of polar peptides EEQYNSTYR with G0F and G2F Glycan modifications at N5 and VTITCSASSR on a Biozen Peptide Polar C18 (Blue), XB-C18 (Green) and PS (Purple) columns and respective Resolution (Rs) values.
| Peptide Type | Peptide | RT | Peak Width | Resolution |
|---|---|---|---|---|
| Peptide Polar C18 | EEQYN(G2F)STYR | 19.95 | 0.16 | |
| EEQYN(GF0)STYR | 20.15 | 0.21 | 1.07 | |
| VTITCSASSR | 22.39 | 0.22 | 10.57 | |
| Peptide XB-C18 | EEQYN(G2F)STYR | 16.45 | 0.21 | |
| EEQYN(GF0)STYR | 16.54 | 0.22 | 0.44 | |
| VTITCSASSR | 18.5 | 0.22 | 8.88 | |
| Peptide PS C18 | EEQYN(G2F)STYR | 17.24 | 0.36 | |
| EEQYN(GF0)STYR | 17.38 | 0.28 | 0.44 | |
| VTITCSASSR | 18.45 | 0.22 | 4.28 |
