Product Information
Phase Detail
Biozen Peptide-PS-C18<br class="t-last-br" />
Mixed-mode C18 column that provides excellent retention for biologics.
Biozen Peptide-PS-C18
Biozen Peptide-PS-C18
Biozen Peptide PS-C18 is a mixed-mode phase that combines a positively charged surface ligand and a C18 ligand. The result is an HPLC column phase that provides excellent retention for biologics.
Separation Mode Reversed Phase |
Pore Size 100 |
Particle Size 1.6, 3 |
Recommended Use Excellent retention of peptides for both peptide mapping and peptide quantitation applications |
Inside bioZen BioTi™ Hardware
Conditions for both columns:
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Column:
Biozen 1.8 μm SEC-3
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Dimension:
150 x 4.6 mm
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Mobile Phase:
50 mM Dipotassium Phosphate + 100 mM
Sodium Sulfate, pH 5.0
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Flow Rate:
0.3 mL/min |
Temperature:
Ambient |
Detection:
UV @ 280 nm |
Sample:
1. γ-Globulin, 5 mg/mL2. Ovalbumin, 1 mg/mL |
Peptide Mapping: Reversed Phase LC
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Column:
 Biozen 1.6 µm Peptide PS-C18 Biozen 2.6 µm Peptide XB-C18 |
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Dimension:
150 x 2.1 mm
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Part No.:
00F-4774-AN
00F-4768-AN |
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Mobile Phase:
A: 0.1 % TFA in Water
B: 0.1 % TFA in Acetonitrile |
Gradient:
Time (min)
0
5 60 65 70 75 78 | % B
1
1 50 50 95 95 95 |
Flow Rate:
bioZen 1.6 µm Peptide PS-C18: 0.3 mL/min bioZen 2.6 µm Peptide XB-C18: 0.6 mL/min |
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Temperature:
40 °C |
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Detection:
UV @ 214 |
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Conditions for all columns:
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Column:
Biozen 2.6 μm Peptide XB-C18 Biozen 1.6 m Peptide PS-C18 |
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Dimension:
150 x 2.1 mm
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Part No.:
00F-4768-AN
00F-4770-AN |
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Mobile Phase:
A: 0.1% Formic Acid in Water
B: 0.1% Formic Acid in Acetonitrile |
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Gradient:
Time (min)
0
5 45 |
% B
3
3 48 |
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Flow Rate:
0.5 mL/min
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Temperature:
50 °C
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Detection:
MS/MS
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Sample:
1. As Noted
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TICs for Infliximab, XB-C18 vs. PS-C18
LC-UV conditions
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Column:
Biozen 2.6 µm Peptide XB-C18
Biozen 1.6 µm Peptide PS-C18 |
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Part No.:
00F-4768-AN (XB-C18)
00F-4770-AN (PS-C18) |
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Mobile Phase:
A. 0.1 % Formic Acid in Water
B. 0.1 % Formic Acid in Acetonitrile |
Gradient:
1-50 % B in 50 minutes |
Flow Rate:
0.3 mL/min |
Detection:
SCIEX® X500B Q-TOF |
Sample:
Infliximab Digest, 1 µg |
Comparative separations may not be representative of all applications.
Conditions for all columns:
Column:
Biozen 1.6 µm Peptide PS-C18 Waters ACQUITY 1.7 µm Peptide CSH |
Dimension:
150 x 2.1 mm |
Part No.:
00F-4770-AN (PS-C18) |
Mobile Phase:
A: 0.1 % Formic Acid in Water B: 0.1 % Formic Acid in Acetonitrile |
Gradient:
1-50 % B int 50 minutes |
Temperature:
50 °C |
Detection:
SCIEX X500B Q-TOF |
Sample:
Trastuzumab Digest, 1 µg |
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