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HPLC
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1061
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biozen-peptide-xb-c18

BioZen Peptide-XB-C18

Digested mAbs or ADCs typically include a large body of compounds which are crucial to understanding post translation modifications. So we designed our Biozen™ Peptide columns to offer highly useful and unique retention profiles. Biozen Peptide XB-C18 allows for fast and effective elution windows by utilizing core-shell particles to gain sharper peaks, better peak capacities, and overall higher sensitivity.

Biozen Peptide columns provide:

  • Unique retention profiles
  • Greater peak coverage
  • High efficiency on both HPLC and UHPLC
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Overview

Inside bioZen BioTi Hardware

Conditions for both columns:

Column:
bioZen 1.8 μm SEC-3
Dimension
150 x 4.6 mm
Flow Rate:
0.3 mL/min
Temperature:
Ambient
Detection
UV @ 280 nm
Mobile Phase:
50 mM Dipotassium Phosphate + 100 mM
Sodium Sulfate, pH 5.0

Sample:

  1. γ-Globulin, 5 mg/mL
  2. Ovalbumin, 1 mg/mL

Peptide Mapping: Reversed Phase LC

Cetuximab Peptide Map

Anti Rituximab Fab Peptide Map

Columns:
bioZen 2.6 μm Peptide XB-C18, bioZen 1.6 m Peptide PS-C18
Dimension
150 x 2.1 mm
Flow Rate:
bioZen 1.6 µm Peptide PS-C18: 0.3 mL/min, bioZen 2.6 µm Peptide XB-C18: 0.6 mL/min
Temperature:
40 °C
Detection
UV @ 214 nm
Mobile Phase:
A: 0.1 % TFA in Water, B: 0.1 % TFA in Acetonitrile
Part No.:
00F-4770-AN, 00F-4768-AN

Gradient:

Time (min)

% B
0
1
5
1
60
50
65
50
70
95
75
95
78
95

Conditions for both columns:

Columns:
bioZen 2.6 μm Peptide XB-C18, bioZen 1.6 m Peptide PS-C18
Dimension
150 x 2.1  mm
Flow Rate:
0.5 mL/min
Temperature:
50 °C
Detection
MS/MS
Mobile Phase:
A: 0.1% Formic Acid in Water, B: 0.1% Formic Acid in Acetonitrile
Part No.:
00F-4768-AN, 00F-4770-AN

Gradient:

Time (min)

% B
0
3
5
3
45
48

Sample:
As noted

TICs for Infliximab, XB-C18 vs. PS-C18

LC-UV conditions

Column:
bioZen 2.6 µm Peptide XB-C18, bioZen 1.6 µm Peptide PS-C18
Flow Rate:
0.3 mL/min
Detection
SCIEX® X500B Q-TOF
Mobile Phase:
A. 0.1 % Formic Acid in Water, B. 0.1 % Formic Acid in Acetonitrile
Part No:
00F-4768-AN (XB-C18), 00F-4770-AN (PS-C18)

Gradient:

Time (min)

1-50 %  B in 50 minutes

Sample:
Infliximab Digest, 1 µg

25942, 25752, 25436
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