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Product Information
Phase Detail
BioZen Peptide-XB-C18

BioZen Peptide-XB-C18

Digested mAbs or ADCs typically include a large body of compounds which are crucial to understanding post translation modifications. So we designed our Biozen™ Peptide columns to offer highly useful and unique retention profiles. Biozen Peptide XB-C18 allows for fast and effective elution windows by utilizing core-shell particles to gain sharper peaks, better peak capacities, and overall higher sensitivity.

Biozen Peptide columns provide:

  • Unique retention profiles
  • Greater peak coverage
  • High efficiency on both HPLC and UHPLC


Separation Mode
Reversed Phase
Pore Size
100, 1000
Particle Size
1.7, 2.6, 5
Recommended Use
Overall retention of both acidic and basic peptides for both peptide mapping and peptide quantitation applications

Inside bioZen BioTi Hardware


Conditions for both columns:
Column:
bioZen 1.8 μm SEC-3
Dimension:
150 x 4.6 mm
Mobile Phase:
50 mM Dipotassium Phosphate + 100 mM
Sodium Sulfate, pH 5.0
Flow Rate:
0.3 mL/min
Temperature:
Ambient
Detection:
UV @ 280 nm
Sample:
1. γ-Globulin, 5 mg/mL
2. Ovalbumin, 1 mg/mL

Peptide Mapping: Reversed Phase LC

Cetuximab Peptide Map

Anti Rituximab Fab Peptide Map


Columns:
bioZen 1.6 µm Peptide PS-C18
 bioZen 2.6 µm Peptide XB-C18
Dimension:
150 x 2.1 mm
Part No.:
00F-4770-AN
00F-4768-AN
Mobile Phase:
A: 0.1 % TFA in Water
B: 0.1 % TFA in Acetonitrile

Gradient:
Time (min)
0
5
60
65
70
75
78
 
% B
1
1
50
50
95
95
95
Flow Rate:
bioZen 1.6 µm Peptide PS-C18: 0.3 mL/min
bioZen 2.6 µm Peptide XB-C18: 0.6 mL/min
Temperature:
40 °C
Detection:
UV @ 214 nm

Conditions for all columns:

Columns:
bioZen 2.6 μm Peptide XB-C18
bioZen 1.6 m Peptide PS-C18
Dimension:
150 x 2.1 mm
Part No.:
00F-4768-AN
00F-4770-AN
Mobile Phase:
A: 0.1% Formic Acid in Water
B: 0.1% Formic Acid in Acetonitrile
Gradient:
Time (min)
0
5
45
 
% B
3
3
48
Flow Rate:
0.5 mL/min
Temperature:
50 °C
Detection:
MS/MS
Samples:
1. As Noted

TICs for Infliximab, XB-C18 vs. PS-C18

LC-UV conditions
Column:
bioZen 2.6 µm Peptide XB-C18
bioZen 1.6 µm Peptide PS-C18
Part No.:
00F-4768-AN (XB-C18)
00F-4770-AN (PS-C18)
Mobile Phase:
A. 0.1 % Formic Acid in Water
B. 0.1 % Formic Acid in Acetonitrile
Gradient:
1-50 %  B in 50 minutes
Flow Rate:
0.3 mL/min
Detection:
SCIEX® X500B Q-TOF
Sample:
Infliximab Digest, 1 µg