HPLC

biozenlc-hplc-column

biozen-wcx

Biozen WCX

Biozen WCX was constructed with linear polycarboxylate chains grafted to monosized non-porous polymeric particles, to sinuously envelop and separate proteins from acidic and basic variants. With a highly tuned and controlled manufacturing process, Biozen WCX affords scientists a way to reproducibly characterize heterogeneity with an added advantage of excellent recovery using bioinert titanium column hardware.

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Overview

bioZen WCX Monosized Polymeric Non-Porous Particle

Monosized particles grafted with linear polycarboxylate chains to envelop and separate proteins from acidic/basic variants

Minimize your priming and promote better recovery

with our BioTi™ biocompatible hardware

Monosized Particles for Optimal Consistency

Our specially crafted polymeric particle is produced with outstanding precision so that you can be confident in increased performance and minimized variation between lots.

Comparative separations may not be representative of all applications.

Columns:

bioZen 6 µm WCX

Dimension

250 x 4.6 mm

Flow Rate:

1 mL/min

Temperature:

30 °C

Detection

UV @ 280 nm

Mobile Phase:

A: CX-1 Gradient Buffer A (pH 5.6), B: CX-1 Gradient Buffer B (pH 10.2)

Part No.:

00G-4777-EO

Gradient:

0-100 % B in 20 minutes

Sample:
Trastuzumab

Note:
CX-1 Gradient Buffers available from Thermo Fisher Scientific™

Charge Variant Analysis

bioZen WCX was crafted to consistently decipher between native protein variants that arise from PTMs within a therapeutics creation and development. The linear polycarboxylate chains grafted to monosized non-porous polymeric particles, envelop and separate proteins from acidic and basic protein variants. With such a highly. tuned and controlled manufacturing process, bioZen WCX media affords scientists a way to reproducibly characterize heterogeneity while taking advantage of excellent recovery through high particle inertness and bioinert titanium BioTi™ column hardware.

Trastuzumab (MES Salt Gradient)

Column:

bioZen 6 μm WCX

Dimension

250 x 4.6 mm

Flow Rate:

1 mL/min

Temperature:

30 °C

Detection

UV @ 280 nm

Mobile Phase:

A: 20 mM MES (pH 5.6), B: 20 mM MES + 300 mM NaCl (pH 5.6)

Part No.:

00G-4777-E0

Gradient:

Time (min)

% B

31.1

100

Sample:
Trastuzumab

Trastuzumab (pH Gradient Buffer)

Column:

bioZen 6 μm WCX

Dimension

250 x 4.6 mm

Flow Rate:

1 mL/min

Temperature:

30 °C

Detection

UV @ 280 nm

Mobile Phase:

A: CX -1 (pH 5.6) pH Gradient Buffer*, B: CX -1 (pH 10.2) pH Gradient Buffer*

Part No.:

00G-4777-E0

Gradient:

Time (min)

% B

100

Sample:
Trastuzumab

* From Thermo Fisher Scientific® Inc.

Acidic and Basic Variant Analysis

Whether using a pH gradient or salt gradient, the bioZen 6μm WCX consistently outperforms ProPac™ WCX-10 columns in charge variant analysis.

Comparative separations may not be representative of all applications.

Columns:

bioZen 6 µm WCX, ProPac 10 µm WCX-10

Dimension

250 x 4.6 mm (bioZen), 250 x 4 mm (ProPac)

Flow Rate:

1.0 mL/min (bioZen), 0.8 mL/min (ProPac)

Temperature:

30 °C

Detection

UV @ 280 nm

Mobile Phase:

A: CX-1 Gradient Buffer A (pH 5.6), B: CX-1 Gradient Buffer B (pH 10.2)

Gradient:

0-100 % B in 20 minutes

Sample:
As indicated

Note:
CX-1 Gradient Buffers available from Thermo Fisher Scientific™

Native Mode Mass Spectrometry with bioZen WCX

Ion-exchange chromatography (IEX) is a technique employed in the characterization of biotherapeutics under nondenaturing conditions. Standard mobile phase conditions for these techniques are high in salt concentration, rendering them incompatible with mass spectrometry (MS) detection. Native MS represents a cutting edge approach to employ MS compatible mobile phases for non-denaturing IEX analysis. Observe and measure the non-covalent interactions and charge variants of proteins at high resolution using bioZen WCX.