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Product Information
Phase Detail
Claricep Spherical C18
Claricep spherical C18 flash columns provide significant performance over traditional flash columns on the market

Claricep Spherical C18

  • Significantly improved performance over regular flash columns
  • Silica deactivated by proprietary process
  • Alternative selectivity for complex purification requirements

Recommended Use
Separation of polar compounds using flash chromatography cartridges

Better Separation Performance

Tetrandrine Extracted from Natural Products, Formulation

Comparative separations may not be representative of all applications.
Flash Conditions

Column A:
Brand X Flash Irregular C18 (40-60 μm 100 Å, 12 g, 3 columns in tandem)
Column B:
Claricep™ Spherical C18 (20-35 μm 100 Å, 12 g, 3 columns in tandem)
Column C:
Claricep Spherical C18 (20 μm 100 Å, 12 g, 3 columns in tandem)
Mobile Phase:
A: Water B: Methanol
Gradient:
Time (min)
0
100
 
% B
85
85
Flow Rate:
83 mL/min
Detector:
UV @ 254/282 nm
Sample
Tetrandrine

Ink Sample Interference / Irregular vs. Spherical

Take 50 μL of two kinds of raw ink samples separately, filter through with a 0.22 μm Nylon Filter and then load onto individual flash columns.

Flash Conditions

Column A:
Claricep™ Irregular C18 (40-60 μm 60 Å, 20 g, 2 columns in tandem)
Column B:
Claricep™ Spherical C18 (40-60 μm 100 Å, 20 g, 2 columns in tandem)
Mobile Phase:
A: Water B: Methanol
Gradient:
Time (min)
0
60
 
% B
10
100
Flow Rate:
26 mL/min
Detector:
UV@254 nm (red), @ 220 nm (blue)
Instrument:
CHEETAH™ MP 200

Polypeptide Isolation

HPLC Analysis

Column:
LC C18, 5 μm, 100 Å, 4.6 × 150 mm
Mobile Phase:
A: Water + 0.01 % TFA
B: Acetonitrile (A/B, 73:27)
Flow Rate:
1 mL/min
Detector:
UV @ 205 nm
Injection Volume:
1 μL

Flash Purification

Column:
Claricep™ C18 (40-60 μm, 100 Å, 12 g)
Part No.:
SO240012-0
Mobile Phase:
Acetonitrile/Water
Flow Rate:
15 mL/min
Detector:
UV @ 205/280 nm
Sample Volume:
2 mL

Purity Test of the Fraction

CLARICEP Spherical C18 Screw-on Columns

Which Screw-on Series should I order ?

CLARICEP i-Series

The i-Series features a managed column head space with a secured screw-on lid. This new design allows either loading of liquid samples directly onto the column head or loading of impregnated solid sample directly into the space. Users will benefit from:

  • Choice of loading method based on sample properties
  • Narrow band for liquid samples because of wide loading area
  • Dry-loading of solid impregnated samples minimizes band broadening
  • Customized loading method upon user preference

Ordering Information
Part Number starts with “SN”. As an example, to order SO240012-0 in i-series, Part Number to order is SN-SO240012-0

I_series

CLARICEP c-Series

The c-Series shares the same design, but the column outlet does not have a luer lock structure, this simplifies tubing connection across various flash systems.

Compatible with instruments from the following:
  • Agela Technologies
  • Buchi
  • Grace
  • Interchim
  • Other brands

Ordering Information
Part Number starts with “C”. As an example, to order SO240012-0 in c-series, Part Number to order is C-SO240012-0

C_series

CLARICEP s-Series

The s-Series columns are fully packed without a head void. In addition, the Luer lock fittings for both inlet and outlet allows easy operation of tandem columns or the coupling of a loading cartridge.

Compatible with instruments from the following:
  • Biotage

Ordering Information
Part Number starts with “S”. As an example, to order SO240012-0 in s-series, Part Number to order is S-SO240012-0

S_series