FLASH

claricep-flash-lc-column

claricep-spherical-c18

Claricep Spherical C18

Significantly improved performance over regular flash columns
Silica deactivated by proprietary process
Alternative selectivity for complex purification requirements

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Overview

Better Separation Performance

Tetrandrine Extracted from Natural Products, Formulation

Comparative separations may not be representative of all applications.

Flash Conditions

Column A:

Brand X Flash Irregular C18 (40-60 μm 100 Å, 12 g, 3 columns in tandem)

Column B:

Claricep™ Spherical C18 (20-35 μm 100 Å, 12 g, 3 columns in tandem)

Column C:

Claricep Spherical C18 (20 μm 100 Å, 12 g, 3 columns in tandem)

Mobile Phase:

A: Water, B: Methanol

Gradient:
Time (min)

% B

100

Flow Rate:

83 mL/min

Detector:

UV @ 254/282 nm

SampleTetrandrine

Ink Sample Interference / Irregular vs. Spherical

Take 50 μL of two kinds of raw ink samples separately, filter through with a 0.22 μm Nylon Filter and then load onto individual flash columns.

Flash Conditions

Column A:

Claricep™ Irregular C18 (40-60 μm 60 Å, 20 g, 2 columns in tandem)

Column B:

Claricep™ Spherical C18 (40-60 μm 100 Å, 20 g, 2 columns in tandem)

Mobile Phase:

A: Water, B: Methanol

Gradient:
Time (min)

% B

100

Flow Rate:

26 mL/min

Detector:

UV@254 nm (red), @ 220 nm (blue)

Instrument:

CHEETAH™ MP 200

Polypeptide Isolation

HPLC Analysis

Column:

LC C18, 5 μm, 100 Å, 4.6 × 150 mm

Flow Rate:

1 mL/min

Detector:

UV @ 205 nm

Mobile Phase:

A: Water + 0.01 % TFA, B: Acetonitrile (A/B, 73:27)

Injection Volume:

1 μL

Flash Purification

Column:

Claricep™ C18 (40-60 μm, 100 Å, 12 g)

Part No.:

SO240012-0

Flow Rate:

15 mL/min

Detector:

UV @ 205/280 nm

Mobile Phase:

Acetonitrile/Water

Sample Volume:

2 mL

Purity Test of the Fraction

CLARICEP Spherical C18 Screw-on Columns

Which Screw-on Series should I order ?

CLARICEP i-Series

The i-Series features a managed column head space with a secured screw-on lid. This new design allows either loading of liquid samples directly onto the column head or loading of impregnated solid sample directly into the space. Users will benefit from:

Choice of loading method based on sample properties
Narrow band for liquid samples because of wide loading area
Dry-loading of solid impregnated samples minimizes band broadening
Customized loading method upon user preference

Ordering Information
Part Number starts with “SN”. As an example, to order CM140012-0 in i-series, Part Number to order is SN-CM140012-0

CLARICEP c-Series

The c-Series shares the same design, but the column outlet does not have a luer lock structure, this simplifies tubing connection across various flash systems.

Compatible with instruments from the following:

Agela Technologies
Buchi
Grace
Interchim
Other brands

Ordering Information
Part Number starts with “C”. As an example, to order CM140012-0 in c-series, Part Number to order is C-CM140012-0

CLARICEP s-Series

The s-Series columns are fully packed without a head void. In addition, the Luer lock fittings for both inlet and outlet allows easy operation of tandem columns or the coupling of a loading cartridge.

Compatible with instruments from the following: