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clarityspe-sample-preparation-products
clarity-rp-desalting
Clarity RP-Desalting
Quick Salt Removal
Clarity RP-Desalting tubes utilize reversed phase, in lieu of size exclusion, to eliminate salt and reagent from trityl off synthetic oligo mixtures in order to capitalize on the speed and loading capacity benefits.
- ~70 % purity and ~80 % recovery of target oligo
- Economical and disposable format
U.S. Patent No. 7,119,145
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Overview
General Extraction
Desalting of Dye-Labeled DNA
A quencher-labeled sample of DNA (25nt) with the sequence FAMTTGACTTAGACTTAGA-CTTAGTTT was desalted using Clarity RP Desalting tubes in the 200 mg/3 mL format. Collection fractions were then analyzed for purity and recovery.
Column:
Clarity 3 µm Oligo-RP C18
Dimension:
50 x 4.6 mm
Flow Rate:
1 mL/ min
Detection:
UV @ 260 nm
Mobile Phase:
A: 50 mM TEAA, pH 7.5 / 5 % Acetonitrile, B: Methanol
Part No.:
00B-4441-E0
Gradient:
A/B (90:10) to A/B (40:60) in 20 min
Sample:
25nt DNA oligonucleotide
Column:
Clarity 3 µm Oligo-RP C18
Dimension:
50 x 4.6 mm
Flow Rate:
1 mL/ min
Detection:
UV @ 260 nm
Mobile Phase:
A: 50 mM TEAA, pH 7.5 / 5 % Acetonitrile, B: Methanol
Part No.:
00B-4441-E0
Gradient:
A/B (90:10) to A/B (40:60) in 20 min
Sample:
40nt DNA
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