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SPE Background

Clarity SPE

Product Information
Clarity sample preparation products provide effective purification for oligonucleotides. They are available in desalting cartridges, as well as reversed phase and ion-exchange selectivities.

The Clarity BioSolutions sample preparation portfolio is composed of unique solid phase extraction chemistries packed in 96-well plate and cartridge formats designed to efficiently and effectively purify oligonucleotides.

  • Works for desalting and clean up of biological samples
  • Specially formulated buffer solutions available for optimal performance
  • Quick protocols for rapid LC-MS/MS results
  • No liquid-liquid extractions (LLE)

Clarity Sample Preparation Products for Synthetic DNA/RNA
Clarity Sample  Prep Suite for Synthetic Oligonucleotide Purification

Oligonucleotide Purification Products

Clarity OTX™ SPE

Clarity OTX™ Extraction Kits

Prior to LC-MS analysis, therapeutic oligonucleotides must be isolated from interferences such as salts, sugars, large proteins, and genomic DNA. Using a mixed-mode solid phase extraction (SPE) sorbent in conjunction with carefully formulated buffers, Clarity OTX consistently delivers high recoveries.


Clarity QSP™ SPE

Clarity QSP™ High-Throughput Purification

Purify trityl-on synthetic DNA or RNA samples in 20 minutes using Clarity QSP 96-well plate and buffer system. Final elution delivers >90 % typical purities/recoveries for in vivo applications and downstream analysis by LC-MS or CE.


Clarity RP-Desalting™

Clarity RP-Desalting™ Quick Salt Removal

Clarity RP-Desalting tubes utilize reversed phase, in lieu of size exclusion, to eliminate salt and reagent from trityl-off synthetic oligo mixtures in order to capitalize on the speed and loading capacity benefits.


Material Characteristics

Clarity Products Particle Support Bonded Phase Particle Shape/Size (μm) Pore Size(Å) Surface Area (m2/g)

Clarity OTX™ SPE

Polymer (surface modified PSDVB)

Mixed-mode anion exchanger




Clarity QSP™ SPE

Polymer (PSDVB)

Hydrophilic polymer coating




Clarity RP-Desalting™






Clarity OTX and Clarity QSP are patented by Phenomenex. U.S. Patent No. 7,119,145

Clarity BioSolutions Product Selection


  Clarity Oligo-RP™ LC Columns Clarity Oligo-MS™ LC Columns
Clarity Oligo-XT LC Columns
Clarity OTX™ LC Columns
Primary Use RP-LC-MS analysis with optimized
selectivity and sensitivity
Rapid, high efficiency RP-LC-MS
analysis for QC and characterization
Extraction of oligo therapeutics from
biological samples for LC-MS bioanalysis
Oligo Length ≤ 60 mer ≤ 60 mer ≤ 40 mer
Recommended Mobile Phase TEA / HFIP TEA/HFIP/MeOH N/A

Characterization / Analysis

  Clarity QSP™ Clarity Oligo-RP™ LC Columns
Clarity Oligo-XT LC Columns
Clarity RP-Desalting™
Primary Use High-throughput, trityl-on RPC
RP-HPLC purification of failure
sequences from target sequences
Quick removal or salt & excess reagent
Purities >90% >90% ~70%
Recoveries ~90% ~70% ~70%
Synthesis Scale Load Up to 50 µmol Up to 50 µmol Up to 1 µmol
Oligo Types DNA, RNA/RNAi, Thioates, Dye-labled, Modified