HPLC
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1014
kinetex-hplc-column
kinetex-polar-c18

Kinetex Core-Shell Polar C18 Column

Increase Polar Selectivity and Performance!

The Kinetex® 2.6 µm Polar C18 is a core-shell based particle with a polar modified surface and a C18 alkyl phase. This versatile column can easily upgrade existing fully porous 5 µm and 3 µm methods to shorten run time, increase sensitivity, and even provide greater resolution with much higher efficiency levels. Combined with being 100 % aqueous stable, the Kinetex Polar C18 is a unique column that combines performance and selectivity.

  • Compatible with All Analytical HPLC/UHPLC Systems*
  • L:1 C18 with Unique Polar Retention
  • Compatible with 100 % Aqueous Mobile Phases
  • USP: L1

*Kinetex analytical columns are compatible with all LC system with standard LC column end-fittings threaded in 10-32. Please contact us for any questions and information about fitting compatibility and any other column internal diameters.

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Overview

C18 and Polar Selectivity

Combined C18 and polar modified surface that provides polar and non-polar retention alongside 100 % aqueous stability.

Increase Polar Retention and Hydrophobic Selectivity

Increase polar selectivity without sacrificing hydrophobic selectivity with the Kinetex Polar C18. The Kinetex Polar C18 is an excellent all-purpose, 100% aqueous stable phase for use with multi-compound mixes that contain polar and non-polar compounds, or even single class methods that have closely related compounds, impurities, or metabolites.

Aromatic Compound Comparison

Conditions for all columns:

Columns:
Kinetex 2.6 µm Polar C18, AQUA 3 µm C18
Dimension:
150 x 4.6 mm
Flow Rate:
1.2 mL/min
Temperature:
22 °C
Detection:
UV @ 210 nm
Mobile Phase:
A: 20 mM Potassium Phosphate (pH 1.5), B: Methanol
Injection Volume:
5 µL
Gradient : Time (min)
% B
0
5
1
30
7
30
7.01
0
14
0

Analytes:

1. Thiamine
2. Nicotinamide
3. Pyridoxal
4. Pyridoxine
5. Pantothenic Acid

Kinetex Where Performance Meets the Right Selectivity

Quickly improve the productivity and performance of your existing methods and utilize the core-shell advantage to deliver performance you need at backpressures the system can handle. Select from a variety of column selectivity to find the right phase for the application.

Polar Compound Phase Comparison

Conditions for all columns:

Columns:
Kinetex 2.6 µm C18, Fully Porous 1.9 µm C18, Kinetex 2.6 µm Polar C18, Kinetex 2.6 µm PS C18
Dimension:
100 x 2.1 mm
Flow Rate:
0.5 mL/min
Temperature:
25 °C
Detection:
MS/MS SCIEX® API 4000™
Mobile Phase:
A: Water with 0.1 % Formic Acid, B: Acetonitrile with 0.1 % Formic Acid
Gradient : Time (min)
% B
0
3
4
100
4.1
3
7
3

Sample:

Acesulfame

Column
Back-Pressure (bar)
RT (min)
Peak Height (cps)
Est. T0* (min)
Kinetex 2.6 µm C18
252
0.67
1.8E+06
0.34
Fully Porous 1.9 µm C18
432
1.07
1.0E+06
0.46
Kinetex 2.6 µm Polar C18
237
1.45
2.1E+06
0.34
Kinetex 2.6 µm PS C18
241
1.85
2.1E+06
0.34
25942,25752,25436

Applications

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