HPLC
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195
kinetex-hplc-column
kinetex-xb-c18

Kinetex XB-C18 Core Shell

A Unique Core-Shell Selectivity

Di-isobutyl side chains differentiate this C18 column. Low ligand density and an inactive surface make this column a great hydrogen bond acceptor. This phase will demonstrate improved peak shape and increased retention of acidic compounds.

  • Compatible with all Analytical HPLC/UHPLC Systems*
  • Unique C18 Retention (USP: L1)
  • Improved Selectivity of Acidic compounds

*Kinetex analytical columns are compatible with all LC system with standard LC column end-fittings threaded in 10-32. Please contact us at Phenomenex.com/chat for any questions and information about fitting compatibility and any other column internal diameters.

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Overview

Selectivity for Acidic Compounds

This unique C18 phase yields increased hydrogen bonding with hydrophobic selectivity‚ resulting in improved peak shape for basic compounds and increased retention of acidic compounds.

Complementary Core-Shell C18 Phases

Ultra-high performance columns like Kinetex will give you very narrow peaks, but without the right selectivities you are left with very narrow, overlapping peaks. The phases offered in the Kinetex column line are complementary to one another, so the spectrum of selectivity your separations require is covered.

C18 Phases (C18 vs. XB-C18)

19487-19488-image

Conditions for all columns:

Dimension:
50 x 2.1 mm
Flow Rate:
0.5 mL/min
Temperature:
30 °C
Detection:
UV @ 220 nm (ambient)
Mobile Phase:
A: Water, B: Acetonitrile
Gradient:Time (min)
% B
0
20
6
60
6.01
20
8
20

Samples:

1. Estriol
2. Hydrocortisone
3. Cortisone
4. Estradiol
5. Cortisone-21-acetate
6. 21-Hydroxyprogesterone
7. Estrone
8. 17-Hydroxyprogesterone
9. Deoxycorticosterone acetate

Manipulate Acidic and Basic Compound Retention

Find the right phase selectivity for your application by investigating the diverse selectivity availability of the Kinetex Core Shell HPLC and UHPLC columns. Where reliable performance meets unique selectivity.

Conditions for all columns:

Dimension:
50 x 2.1 mm
Flow Rate:
0.8 mL/min
Temperature:
30 °C
Detection:
UV @ 245 nm (ambient)
Mobile Phase:
A: Water with 0.1% Formic Acid, B: Acetonitrile with 0.1% Formic Acid
Gradient:Time (min)
% B
0
5
0.2
5
4.2
95
4.21
5
5.5
5

Samples:

1. Pyridine
2. Acetaminophen
3. Pindolol
4. Quinidine
5. Sulfathiazole
6. Acebutolol
7. Benzyl alcohol
8. Chlorpheniramine
9. Phenol
10. Triprolidine
11. Nortriptyline
12. Prednisolone
13. 3-Methyl, 4-nitrobenzoic acid
14. 2-Hydroxy, 5-methylbenzaldehyde
15. Diflunisal
16. Hexanophenone

25942,25752,25436

Applications

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