Phenogel 100 Å GPC/SEC Columns

The Optimal Choice for Aqueous and Mixed Organic Solutions

Molecular Weight Range: 500 - 6 K
Recommended for use with small organics
Wide range of solvent compatibility: packed in tetrahydrofuran (THF); additional shipping solvents (i.e. DMF, Toluene, Chloroform, and others) available on request
Temperature stable to 140 °C
Reduce solvent consumption and disposal costs up to 65 % while retaining elution profile with Narrow Bore columns
Recommended column for USP L21

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HPLC

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Applications on Phenogel Fixed Pore 100Å Non-Aqueous GPC/SEC Columns

Polyethylene Glycol 330

USP Hydrocortisone

Columns:

Phenogel 5 µm 50 Å, 100 Å, 500 Å

Dimension:

300 x 7.8 mm

Part No.:

00H-0441-K0 (50 Å)
00H-0442-K0 (100 Å)
00H-0443-K0 (500 Å)

Solvent:

THF

Flow Rate:

1.0 mL/min

Temperature:

Ambient

Injection Volume:

100 µL 0.25% w/v

Detection:

Differential Refractometer

Sample:

1. dp7 546 MW
2. dp6 458 MW
3. dp5 370 MW
4. dp4 282 MW
5. dp3 226 MW
6. dp2 106 MW

7.dp1 62 MW

USP Hydrocortisone

Columns:

Phenogel 5 µm 50 Å, 100 Å, 500 Å

Dimension:

300 x 7.8 mm

Part No.:

00H-0441-K0 (50 Å)
00H-0442-K0 (100 Å)
00H-0443-K0 (500 Å)

Solvent:

THF

Flow Rate:

1.0 mL/min

Temperature:

Ambient

Injection Volume:

100 µL 0.25% w/v

Detection:

Differential Refractometer

Sample:

1. C40 562 MW
2. C32 450 MW
3. C24 338 MW
4. C20 482 MW
5. C16 226 MW
6. C13 684 MW

The columns were used in tandem to characterize isoprene chain lengths removed from labeled in vitro translation products or cell proteins. The isoprenoids were removed by treatment with Raney nickel and extracted into pentane. The pentane extractable material was hydrogenated over platinum catalyst and injected onto the column. Fractions were collected at 0.5 minute intervals and radioactivity was monitored by liquid scintillation. These saturated hydrocarbon chains were characterized by comparing radioactive peaks to standard retention times.

Isoprenes from In Vitro Translation on Products

USP Hydrocortisone

Columns:

Phenogel 5 µm 50 Å, 100 Å in series

Dimension:

300 x 7.8 mm

Part No.:

00H-0441-K0 (50 Å)
00H-0442-K0 (100 Å)

Solvent:

THF

Flow Rate:

0.25 mL/min

Temperature:

Ambient

Detection:

Differential Refractometer

Sample:

1. Squalene C30
(2, 6, 10, 15, 19, 23-Hexamethyltetracosane)
2. Phytane C20
(2, 6, 10, 14-Tetramethylhexadane)
3. Farnesne C15
(2, 6, 10-Trimethyldodecane)

Environmental and Biomonitoring

An application of preparative GPC columns for the automated purification of environmental samples in concordance to the 3640A EPA method. Results shown are the analysis and purification of PAHs by Phenogel GPC columns and the removal of biomaterial matrix interferences that are known to complicate the analysis of PAHs in biological tissues. This method showed that Phenogel 5 µm 100 Å preparative GPC columns efficiently removed lipids from salmon and mussel samples with high recovery of the target 49 PAHs while demonstrating good repeatability and that it could easily be automated for overnight injection.

Columns:

Phenogel 5 μm 100 Å

Dimension:

300 x 21.2 mm

Part No.:

00H-0442-P0

Mobile Phase:

Methylene chloride

Flow Rate:

5 mL/min

Detector:

Agilent® 1100

Oligomer Composition of Over-the-Counter Omega-3 Fish Oil Capsules

Among the variety of over-the-counter (OTC) vitamin and nutraceutical products sold to consumers, fish oils containing omega-3 fatty acids are some of the most widely endorsed by healthcare professionals. Three different OTC fish oil supplements were analyzed by GPC chromatography to determine oligomer composition.

One of the three samples analyzed had an oligomer content that would not have met the acceptance criteria of the Ph. Eur. monograph. Further analysis would need to be performed to characterize the exact composition of omega-3 fatty acids in sample OTC-1.