Phenogel 500 Å GPC/SEC Columns

The Optimal Choice for Aqueous and Mixed Organic Solutions

Molecular Weight Range: 1 K - 15 K
Recommended for use with small organics
Wide range of solvent compatibility: packed in tetrahydrofuran (THF); additional shipping solvents (i.e. DMF, Toluene, Chloroform, and others) available on request
Temperature stable to 140 ˚C
Reduce solvent consumption and disposal costs up to 65% while retaining elution profile with 500 Å Narrow Bore columns

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HPLC

2552

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phenogel-500a

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Applications on Phenogel Fixed Pore 500 Å Non-Aqueous GPC/SEC Columns

Phenolic Resins

USP Hydrocortisone

Columns:

Phenogel 5 µm 500 Å x 2, 10³ Å, 10⁴ Å

Dimension:

300 x 7.8 mm

Part No.:

00H-0443-K0 (500 Å)
00H-0444-K0 (10³ Å)
00H-0445-K0 (10⁴ Å)

Solvent:

THF

Flow Rate:

1 mL/min

Temperature:

25 °C

Detection:

Sample:

Phenolic Aldehyde Resin, MW 500 to 470 K

Polyethylene Glycol 330

USP Hydrocortisone

Columns:

Phenogel 5 μm 50 Å, 100 Å, 500 Å

Dimension:

300 x 7.8 mm

Part No.:

00H-0441-K0 (50 Å)
00H-0442-K0 (100 Å)
00H-0443-K0 (500 Å)

Solvent:

THF

Flow Rate:

1 mL/min

Temperature:

25 °C

Detection:

Differential Refractometer

Sample:

1. dp7 546 MW
2. dp6 458 MW
3. dp5 370 MW
4. dp4 282 MW
5. dp3 194 MW
6. dp2 106 MW
7. dp1 62 MW

USP Hydrocortisone

Columns:

Phenogel 5 μm 50 Å, 100 Å, 500 Å

Dimension:

300 x 7.8 mm

Part No.:

00H-0441-K0 (50 Å)
00H-0442-K0 (100 Å)
00H-0443-K0 (500 Å)

Solvent:

THF

Flow Rate:

1 mL/min

Temperature:

25 °C

Detection:

Differential Refractometer

Sample:

1. C40 562 MW
2. C32 450 MW
3. C24 338 MW
4. C20 282 MW
5. C16 226 MW
6. C13 184 MW

Poly-(α-Methyl Styrene) (Wide MW Range)

USP Hydrocortisone

Columns:

Phenogel 5 μm 50 Å, 100 Å, 500 Å

Dimension:

300 x 7.8 mm

Part No.:

00H-0446-K0 (10⁵ Å)
00H-0445-K0 (10⁴ Å)
00H-0444-K0 (10³ Å)
00H-0443-K0 (500 Å)

Solvent:

THF

Flow Rate:

1 mL/min

Detection:

Differential Refractometer

Injection Volume:

100 µL 0.25 % w/v

Sample:

1. 680,000 MW
2. 90,000 MW
3. 30,000 MW
4. 6,000 MW

Polymethyl Methacrylates (Wide MW Range)

USP Hydrocortisone

Columns:

Phenogel 5 µm 10⁵ Å, 10⁴ Å, 10³ Å, 500 Å

Dimension:

300 x 7.8 mm

Part No.:

00H-0446-K0 (10⁵ Å)
00H-0445-K0 (10⁴ Å)
00H-0444-K0 (10³ Å)
00H-0443-K0 (500 Å)

Solvent:

THF

Flow Rate:

1 mL/min

Detection:

Differential Refractometer

Injection Volume:

100 µL 0.25 % w/v

Sample:

1. 700,000 MW
2. 107,000 MW
3. 27,000 MW

Polybutadienes (Wide MW Range)

USP Hydrocortisone

Columns:

Phenogel 5 µm 10⁵ Å, 10⁴ Å, 10³ Å, 500 Å

Dimension:

300 x 7.8 mm

Part No.:

00H-0446-K0 (10⁵ Å)
00H-0445-K0 (10⁴ Å)
00H-0444-K0 (10³ Å)
00H-0443-K0 (500 Å)

Solvent:

THF

Flow Rate:

1 mL/min

Detection:

Differential Refractometer

Injection Volume:

100 µL 0.25 % w/v

Sample:

1. 420,000 MW
2. 24,000 MW
3. 2,500 MW
4. 500 MW

Polyvinyl Butyral

USP Hydrocortisone

Columns:

Phenogel 5 μm 50 Å, 100 Å, 500 Å

Dimension:

300 x 7.8 mm

Part No.:

00H-0441-K0 (50 Å)
00H-0442-K0 (100 Å)
00H-0443-K0 (500 Å)

Solvent:

THF

Flow Rate:

1 mL/min

Temperature:

Ambient

Detection:

Differential Refractometer

Injection Volume:

100 μL 0.25% w/v

Sample:

1. dp7 546 MW
2. dp6 458 MW
3. dp5 370 MW
4. dp4 282 MW
5. dp3 194 MW
6. dp2 106 MW

6. dp1 62 MW

Oligomer Composition of Over-the-Counter Omega-3 Fish Oil Capsules

Among the variety of over-the-counter (OTC) vitamin and nutraceutical products sold to consumers, fish oils containing omega-3 fatty acids are some of the most widely endorsed by healthcare professionals. Three different OTC fish oil supplements were analyzed by GPC chromatography to determine oligomer composition.

One of the three samples analyzed had an oligomer content that would not have met the acceptance criteria of the Ph. Eur. monograph. Further analysis would need to be performed to characterize the exact composition of omega-3 fatty acids in sample OTC-1.