Rezex ROA-Organic Acid H+ (8%) Ion Exclusion HPLC Columns
- 8% cross-linked hydrogen
- For use with organic acids in combination with carbohydrates, alcohols, fatty acids, neutral compounds; amino sugars
- Uniquely suited to monitor starches, sugars, organic acids, and ethanol in one run for bioethanol fermentation analysis
- Achieve excellent base line separation between Dp3 and Dp4+
HPLC
80
296
rezex-hplc-column
rezex-roa-organic-acid-h
smooth-scroll-vertical-tabs
Overview
Cross Reference Guide
Rezex
Description |
Applications
Bio-Rad® Aminex®
Supelco® Supelcogel™
Waters® Sugar-Pak™
Transgenomic® CARBOSep™
Sepax® Carbomi®
ROA-Organic Acid (USP L22 packing)*
8 % cross-linked HYDROGEN
– Organic acids alone
– Organic acids in combination with carbohydrates, alcohols, fatty acids, or neutral compounds
– Ethanol, acetic acid, glycerol, and standard alcohol mixtures
– Amino sugars
– Organic acids in combination with carbohydrates, alcohols, fatty acids, or neutral compounds
– Ethanol, acetic acid, glycerol, and standard alcohol mixtures
– Amino sugars
HPX-87H
125-0140
125-0140
Supelcogel
C-610H & H
C-610H & H
N/A
N/A
N/A
Sharper Peak Shape = Easy & Accurate Quantitation
Organic Acids in Wine
Columns:
Rezex ROA-Organic Acid Aminex HPX-87H
Dimension:
300 x 7.8 mm
Flow Rate:
0.5 mL/min
Temperature:
40 °C
Detection:
UV @ 210 nm
Mobile Phase:
0.005 N H2SO4
Sample:
1. Citric acid
2. Tartaric acid
3. Succinic acid
4. Lactic acid
5. Acetic acid
Lower Backpressure = Longer Column Lifetimes and Faster Run Time Capability
Aliphatic Acids
Aminex and Bio-Rad are registered trademarks of Bio-Rad Laboratories, Inc. Comparative separations may not be representative of all applications.
Columns:
Rezex ROA-Organic Acid Aminex HPX-87H
Dimension:
300 x 7.8 mm
Flow Rate:
0.5 mL/min
Temperature:
40 °C
Detection:
UV @ 210 nm
Mobile Phase:
0.005 N H2SO4
Sample:
1. Oxalic acid
2. Citric acid
3. Tartaric acid
4. Succinic acid
5. Formic acid
6. Acetic acid
Food and Beverage
White Wine
Columns:
Rezex ROA-Organic Acid Aminex HPX-87H
Dimension:
300 x 7.8 mm
Flow Rate:
0.5 mL/min
Temperature:
40 °C
Detection:
UV @ 210 nm
Mobile Phase:
0.005 N H2SO4
Sample:
1. Oxalic acid
2. Citric acid
3. Tartaric acid
4. Succinic acid
5. Formic acid
6. Acetic acid
Bioethanol
Columns:
Rezex ROA-Organic Acid
Dimension:
300 x 7.8 mm
Flow Rate:
0.6 mL/min
Temperature:
60 °C
Detection:
Shimadzu® Prominence® LC-20A System
Mobile Phase:
0.005 N Sulfuric Acid
Part No.:
System:
RI @ 40 °C
Sample:
1. Dp4+
2. Dp3
3. Maltose
4. Glucose
5. Lactic Acid
6. Glycerol
7. Acetic Acid
8. Ethanol
Columns:
Rezex ROA-Organic Acid
Dimension:
150 x 7.8 mm
Flow Rate:
0.6 mL/min
Temperature:
60 °C
Detection:
Shimadzu® Prominence® LC-20A System
Mobile Phase:
0.005 N Sulfuric Acid
Part No.:
System:
RI @ 40 °C
Sample:
1. Dp4+
2. Dp3
3. Maltose
4. Glucose
5. Lactic Acid
6. Glycerol
7. Acetic Acid
8. Ethanol
Saccharides
Monosaccharides with Organic Acids
Columns:
Rezex ROA-Organic Acid
Dimension:
300 x 7.8 mm
Flow Rate:
0.5 mL/min
Temperature:
75 °C
Mobile Phase:
0.1 % formic acid
Part No.:
System:
ELSD
Sample:
1. Citric acid
2. Tartaric acid
3. Glucose
4. Fructose
5. Succinic acid
Alcohols
Carboxylic Acids
Columns:
Rezex ROA-Organic Acid
Dimension:
300 x 7.8 mm
Flow Rate:
1.0 mL/min
Temperature:
40 °C
Mobile Phase:
Water + 0.5 % Trifluoroacetic Acid
Part No.:
System:
RI
Sample:
1. Acetylene Carboxylic Acid
2. Maleic Acid
3. Succinic Acid
4. Fumaric Acid
Monosaccharides with Organic Acids
Columns:
Rezex ROA-Organic Acid
Dimension:
300 x 7.8 mm
Flow Rate:
0.6 mL/min
Temperature:
Ambient
Mobile Phase:
1 % Phosphoric Acid
Part No.:
System:
RI
Sample:
1. Glucose
2. N-Acetylglucose
3. N-Acetylgalactosamine
Applications (partial list)
Call us for assistance in selecting the right column for your needs!
Compound
App ID No.
Acetic Acid
5515
Acetylene Carboxylic Acid
5517
Ethanol
5512
Formic Acid
5512
Fumaric Acid
5517
Glucose
5514
Isopropanol
5512
Maleic Acid
5517
Compound
App ID No.
Methanol
5512
n-Butanol
5512
n-Propanol
5512
n-Acetylgalactosamine
5514
n-Acetylglucose
5514
Oxalic Acid
5515
sec-Butanol
5512
Succinic Acid
5515
Specifications and Operating Recommendations
RAM Carbohydrate
Part No.
Ionic Form
Hydrogen |
Standard Dimensions
300 x 7.8 mm
Matrix
Sulfonated Styrene Divinyl Benzene
Cross Linking
8 %
Particle Size (μm)
8
Min. Efficiency (p/m) based on last peak
50,000 (Acetic Acid)
Typical Pressure (psi @ Testing Flow Rate)
580
Max. Pressure (psi @ Max Flow Rate)
1,000
Max. Flow Rate (mL/min)
1.0
Max. Temperature (°C)
85
Typical Mobile Phase
0.005 N H2SO4
pH Range
1-8 |
Guard Column Part
Cleaning, Regeneration and Storage
Organic Modifiers (Max)
5 % Methanol, IPA, EtOH
Inorganic Modifiers
5 % HNO3, H3PO4
Avoid
Acids, Bases, Salts or Metal Ions, pH > 3, > 30 % Acetonitrile
Cleaning Solvent
100 % Water
Flow Rate(mL/min)
0.4
Temperature (°C)
85
Duration (hrs)
12
Regeneration Solvent
0.025 M H2SO4
Flow Rate (mL/min)
0.2
Duration (hrs)
4-16
Ship/Storage Solvent
0.005 N H2SO4
Cross Reference Guide
Documents
View All Documents