search
Product Information
Phase Detail
Strata-XL-CW SPE Products
Strata-X-L-CW solid phase extraction (SPE) sorbent is a polymeric large particle weak cation exchange (WCX) phase SPE sorbent for viscous samples
Strata-XL-CW SPE Products
Strata-XL-CW SPE Products
Large Particle Weak Cation Mixed-Mode Solid Phase Extraction (SPE)
A weak cation-exchange functionalized polymeric sorbent that allows for complete retention of basic compounds with a pKa greater than 8, including quaternary amines, making 100% organic wash conditions possible. A 100% organic wash ensures that the maximum amount of interferences are removed from the target compound. The large particle format is ideal for processing large volumes or viscous samples.
Brand Strata-X |
Recommended Use Extraction of basic compounds, particularly strong bases, present in viscous samples |
3 Mechanisms of Retention
Weak Cation-Exchange
Ï€-Ï€ Bonding
Hydrophobic Interaction
Available Formats
96-Well-Plate
1, 3, and 6mL Tubes
Giga Tubes (12-150mL Tubes)
Starting Method
For Bases with pKa > 8
|
Column:
1 mL Methanol
|
|
Equilibrate:
1 mL Water, pH 6-7
|
|
Load:
Diluted Sample pH 6-7
|
|
Wash 1:
1 mL Water, pH 6-7
|
|
Wash 2:
1 mL Methanol (collect this fraction to analyze Neutrals/Acids)
|
|
Elute Any Acids:
2x 500 µL 5 % Formic Acid in Methanol
|
|
Elute Weak Acids:
2x 500 µL 5 % NH4OH in Methanol
|
Based on 30 mg/1 mL sorbent mass. The above is a convenient starting point for SPE method development, further optimization may be required to tailor the method for your specific extraction needs.
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide.
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide.