USP Alosetron Hydrochloride Assay and Organic Impurities on Gemini 3 µm C18 (AN-1010)
This application note for the LC-UV assay and organic impurities for Alosetron hydrochloride is based on the USP monograph, which was validated using the Gemini 3 µm C18 column for both the assay (isocratic) and organic impurities (gradient).
USP Alosetron Tablets Assay and Organic Impurities on Gemini 3 µm C18 (AN-1011)
Alosetron hydrochloride, a potent and selective antagonist of the serotonin 5-HT3 receptor, is the active pharmaceutical ingredient in Lotronex tablets, and there are several generic versions of Alosetron tablets available on the market. This application note for the LC-UV assay and organic impurities for Alosetron tablets is based on the official USP monograph, which was validated using the Gemini 3 µm C18 column for both the assay (isocratic) and organic impurities (gradient).
The Alternative Matrix Guide: Hair and Oral Fluid Method Development
Both hair and oral fluid are difficult matrices to work with due to the sample preparation and chromatography challenges. Discover our method development solutions for these alternative matrices and how to solve these problems in your own lab.
USP Vardenafil Hydrochloride Assay and Organic Impurities on Gemini 5 µm C18 and Gemini 5 µm NX-C18 (AN-1014)
This application note for the LC-UV assay and organic impurities for Vardenafil hydrochloride drug substance is based on the official USP monograph which calls for an L1 (C18) 5 µm, 250 x 3.0 mm column under gradient conditions for both assay and organic impurities. The mobile phase, standard solution, and system suitability solution for the assay and organic impurities were prepared in accordance with the USP monograph, using the USP Vardenafil hydrochloride reference standard and Vardenafil hydrochloride system suitability reference standard (containing 99% Vardenafil hydrochloride and 1% 7-Methylvardenafil). Vardenafil hydrochloride is a PDE-5 inhibitor and is the generic version of the brand name drug Levitra.
Cannabis Testing Guide (LC/GC/SP)
The latest applications and resources for testing potency, pesticides, mycotoxins, terpenes, and residual solvents.
Vitamin A and E from Human Serum using Novum SLE (TN-0095)
Enhancing the extraction of Vitamin A and E in serum using Simplified Liquid Extraction (SLE) significantly improves the LC-MS/MS detection of target compounds, all while preserving a simple and robust method.
Performance of Surface Modified Luna Omega and Kinetex PS Columns for the Separation of Tricyclic Antidepressants
Performance of Surface Modified Luna Omega and Kinetex PS Columns for the Separation of Tricyclic Antidepressants
Optimization of Elution Solvents for Simplified Liquid Extraction of THC and Metabolites from Urine (TN-0112)
In this technical note, we develop a simplified liquid extraction (SLE) application for THC and metabolites in urine in order to identify the optimum elution solvent from 7 different elution solvent variants. Results from each extraction were then compared based on recovery as well as a general baseline cleanliness in an effort to understand which solvent(s) is most suitable for this analysis.
Reproducible FAME Analysis on 100 meter Zebron ZB-FAME GC Column
Reproducible FAME Analysis on 100 meter Zebron ZB-FAME GC Column
Determination of Glyphosate and AMPA According to DIN ISO 16308
Glyphosate is the most widely used herbicide globally and is of priority concern with many food and environmental agencies. Presented is an FMOC (Fluorenylmethyloxycarbonyl chloride) derivatization method using a reversed phase procedure.
An Analytical Method Using the Zebron™ ZB-1 GC Column to Highlight the Opportunity to Resolve the Volatile Polar Sulfur Compounds in Petroleum Fuel
An Analytical Method Using the Zebron™ ZB-1 GC Column to Highlight the Opportunity to Resolve the Volatile Polar Sulfur Compounds in Petroleum Fuel
Faster Analysis of 18 Phthalates by GC-MS using Zebron ZB-PAH-CT GC Column
Faster Analysis of 18 Phthalates by GC-MS using Zebron ZB-PAH-CT GC Column
Luna Omega C18 Application eBook
One of the world's leading HPLC brands, now enhanced for incredible LC performance! Luna Omega 1.6µm, 3µm, and 5µm C18 HPLC/UHPLC columns culminate 20 years of technological advancements and innovation.
Reference Analysis of Loratadine from an Oral Solution Using a Kinetex Biphenyl HPLC/UHPLC Column
Loratadine is an antihistamine that can be administered through an oral solution. Because of the aromatic nature of the compound, we investigated a reversed phase method that leveraged the Kinetex 5 µm Biphenyl column. This column was selected because of its relative increased polar retention and aromatic selectivity
Chromatographic Considerations for LC-MS/MS Analysis of Amphetamine in the Presence of Gabapentin using Kinetex Core-Shell LC Columns (TN-1247)
Since gabapentin is unmetabolized and excreted renally, it is found in high concentrations in urine. These high concentrations can make analysis of other compounds, specifically amphetamine, difficult due to overloading the LC column and saturating the source of the mass spectrometer. This work examines the most commonly used drug research panel method conditions and evaluate modifications that can be made to alleviate these issues for robust analysis of amphetamine in a high concentration gabapentin positive sample.
Successful Separation of Cannabidiol Enantiomers by LC-UV Analysis using Lux Amylose Chiral Columns
Cannabidiol (CBD) is a phytocannabinoid found in Cannabis, and a major focus of both pharmaceutical research and consumer products. CBD formed from within the plant is created by an enzyme-mediated process which exclusively forms the levorotatory isomer, (-)-cannabidiol, as has been confirmed by optical rotation and NMR analysis of plant extracted and purified material. The first synthetic approach to cannabinoids was based on the use of commercially available, chiral starting materials. This approach is still used in many pharmaceutical synthetic processes this day. For the development of plant-based cannabinoid products, having the ability to verify enantiomeric purity would be an excellent opportunity for quality verification of their products.
GC Troubleshooting Guide
The complete guide to GC troubleshooting with hints, tips, and tricks for chromatographic remedies.
Choosing the Right UHPLC Column for Highly Polar Basic Compounds
This technical note walks through the various considerations that need to be taken into account when working with polar basic compounds. Several UHPLC columns are compared including a Luna Omega Polar C18 and a Waters ACQUITY BEH C18 column.
Determination of Pantothenic Acid (Vitamin B5) in Human Serum by LC-MS/MS using a Luna Omega Polar C18 column
In this application note, we describe a simplified extraction method of Vitamin B5 from human serum followed by an LC-MS/MS method using a Luna Omega 3 µm Polar C18 column.
Choosing the Right UHPLC Column for Intact Peptide Analysis
This technical note walks through the various considerations that need to be taken into account when performing intact peptide analysis. Several UHPLC columns are compared including a Luna Omega C18 and PS C18 and a Waters ACQUITY BEH C18 column.
Choosing the Right UHPLC Column for Peptide Mapping
Analysts are increasingly relying upon LC-MS to perform their peptide and protein digest analyses, and TFA is not often used in conjunction with LC-MS due to its strong signal-suppression properties. Thus, when performing peptide analysis by LC-MS using typical mobile phases such as water/acetonitrile/formic acid, secondary interactions between basic amino acids on the peptide and the underlying silica can lead to broad peaks, peak tailing, and can interfere with optimal resolution between close-eluting peaks. To obtain optimal performance for peptide analysis using either LC-UV or LC-MS, the ideal media should be highly efficient (narrow peaks) and highly inert (minimal peak tailing when using MS-compatible modifiers like formic acid). In addition, a more retentive media is often favorable, particularly for peptide mapping, to maximize retention of small, polar peptide fragments that may not have a high affinity for a typical C18 phase, particularly in the absence of TFA (trifluoroacetic acid). Using this information, Luna Omega PS C18 and C18 were tested alongside a Waters ACQUITY UHPLC column.
LC-MS/MS Separation of Furosemide and its Metabolite Using the Kinetex 2.6 µm Biphenyl, Luna Omega 1.6 µm C18, and Luna Omega 1.6 µm Polar C18 Columns
LC-MS/MS Separation of Furosemide and its Metabolite Using the Kinetex 2.6 µm Biphenyl, Luna Omega 1.6 µm C18, and Luna Omega 1.6 µm Polar C18 Columns
Analysis of Explosives Found in EPA 8330A and 8330B Using a Kinetex 2.6 µm Polar C18 Column
Here we present a single high efficiency separation and analysis of common explosives, typically analyzed by EPA 8330A and 8330B. For such a mixture of related compounds, the Polar C18 stationary phase bonded to a Kinetex core-shell particle was chosen because of its high efficiency separation power alongside dual polar/non-polar selectivity that can greatly help to magnify small differences between similar compounds of interest.
Choosing the Right UHPLC Column for General Pharmaceutical Screening
This technical note walks through the various considerations that need to be taken into account when performing a general screening of pharmaceutical compounds. Several UHPLC columns are compared including a Luna Omega C18 and PS C18 and a Waters ACQUITY BEH C18 column.
Choosing the Right UHPLC Column for Multi-Class LC-MS/MS Screening - Pesticides Panel
This technical note walks through the various considerations that need to be taken into account when performing multi-class screening such as a pesticide panel. Several UHPLC columns are compared including a Luna Omega C18 and Polar C18 and a Waters ACQUITY BEH C18 column.
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