UHPLC/HPLC Method Transfer Tool

Welcome to your UHPLC/HPLC Method Transfer Tool!

As you develop a new method or take on an existing method, it can be time consuming to figure out the exact adjustments needed to transfer said method across different instrumentation, particle morphologies or even LC particle sizes. We've definitely felt that pain over the years and created this Method Transfer Tool to allow you to take back that time!

Enjoy!

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LC Instrument 1Predicted Total Pressure (bar)LC Instrument 2Predicted Total Pressure (bar)
Pressure Limit (bar) =436Pressure Limit (bar) =397
Delay Volume (mL) =

Typical Delay Volume Values:

1.100 mL Agilent 1100/1260 Binary

1.000 mL Agilent 1100/1260 Quaternary

0.450 mL Agilent 1290 Quaternary

0.450 mL Waters ACQUITY H-Class

0.200 mL Agilent 1290 Binary

0.125 mL Waters ACQUITY UPLC/I-Class

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Delay Volume (mL) =

Typical Delay Volume Values:

1.100 mL Agilent 1100/1260 Binary

1.000 mL Agilent 1100/1260 Quaternary

0.450 mL Agilent 1290 Quaternary

0.450 mL Waters ACQUITY H-Class

0.200 mL Agilent 1290 Binary

0.125 mL Waters ACQUITY UPLC/I-Class

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Instrument Type =Instrument Type =
Maximum Viscosity =ACN / H2O 25CSeparation Goal =
LC Column 1LC Column 2
Length (mm)Column ID (mm)Flow Rate (mL/min)Particle Type / Size (μm)PorosityLength (mm)Column ID (mm)Flow Rate (mL/min)Particle Type / Size (μm)Porosity

5 μm Core-Shell

5 μm Fully Porous

4 μm Fully Porous

3.6 μm Core-Shell

3.5 μm Fully Porous

3 μm Fully Porous

2.6 μm Core-Shell

1.7 μm Core-Shell

1.6 μm Fully Porous

1.3 μm Core-Shell

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0.5250.45

5 μm Core-Shell

5 μm Fully Porous

4 μm Fully Porous

3.6 μm Core-Shell

3.5 μm Fully Porous

3 μm Fully Porous

2.6 μm Core-Shell

1.7 μm Core-Shell

1.6 μm Fully Porous

1.3 μm Core-Shell

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0.525
Original Gradient TableScaled Gradient Table
Time (min)%A%B#CV%B/CVTime%A%B#CV%B/CV
013.300.009911.37
111.732.7699113.66
1311.731.025.13871311.731.02
9546.931.7514.6159546.931.75
955.8715.805955.87
9525.2816.885955.37
1 Column Volume (mL) =0.1361 Column Volume (mL) =0.091
Min Equilibration Time (min) =1.244Min Equilibration Time (min) =1.086
Delay Time (min) =0.563Delay Time (min) =0.278
Void Time (min) =0.170Void Time (min) =0.202
Linear Velocity (mm/s) =7.332Linear Velocity (mm/s) =4.125
Max Instrument Delay Volume (mL) =1.367
Injection Delay Time (min) =NaN