Our team of experts understands the challenges associated with Clinical Research; starting at the sample itself all the way through separation and analysis. Let us help you boost your throughput and improve your workflows with our portfolio of Sample Preparation and LC products that are sure to fit your needs.
High-efficiency core-shell product (1.7, 2.6 or 5 µm) capable of adding extra separation power to your analysis of non-polar and polar compounds. Take advantage of multiple selectivity interaction mechanisms such as hydrophobic, pi-pi, and dipole-dipole to improve tough separations.
Faster, Easier, More Reliable: SLE Simplified
New Novum PRO SLE additionally gets down to low (LLOD) extraction levels from plasma with added clean manufacturing steps.
New reversed phase polymer with matrix removal technology offers a faster, cleaner way to perform SPE. Learn how to reduce protocol time by at least 40%.
Separation of Low Level Estrogen Analytes from Plasma by LC-MS/MS Analysis in Conjunction with Novum PRO Simplified Liquid Extraction (SLE)
Quantitation of Pain Panel Analytes from Oral Fluid Utilizing Microelution Solid Phase Extraction Coupled with LC-MS/MS
Maximizing Recovery of 25-OH Vitamin D2 and D3 using Phospholipid Removal Plates and Core-Shell LC-MS/MS Analysis
Optimization of Chromatography for Intact Mass Analysis of Monoclonal Antibodies Using Biozen WidePore C4 Columns
How do you pretreat a whole blood sample for SPE?
A simple pretreatment is to add 10% TCA (trichloroacetic acid) aqueous solution in the ratio of 1:1.
Centrifuge and collect the supernatant. Another simple way to pretreat whole blood is to perform protein precipitation with cold acetonitrile-methanol (85:15) in the ratio of 1:2. After centrifuging, the supernatant is diluted with solvent appropriate for extraction loading.A more thorough pretreatment is converting whole blood into blood plasma. Centrifuging anti-coagulated blood gives blood plasma as supernatant. Blood plasma preparation kits with prepackaged tubes containing an anticoagulant are commercially available.
For more information on sample pretreatment for extraction using Strata and Strata-X, please contact us.
I have seen you have a number of different applications for Vitamin D, which ones should I consider?
When attempting to analyze vitamin D, it is important to consider the source of the sample.That is, if samples are from a biological source the vitamin D is likely to be present as the 25-hydroxyvitamin D3 . The difficulty will be in separation of 25-hydroxy and 3-epi-25-hydroxy vitamin D3, and we have applications specific for this critical pair.If the separation is vitamin D from a food stuff or supplement, it will be present just as Vitamin D3 (cholecalciferol) and this is a much more straightforward separation. We have applications on this as well as other fat-soluble vitamins.