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Clinical Research
Phenomenex provides everything you need for clinical research, from sample prep to LC-MS analysis. Improve workflows with our user-friendly products and expert support.
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Clinical Research

From Clinical Sample Preparation to LC-MS Analysis, We Have You Covered

Our team of experts understands the challenges associated with Clinical Research; starting at the sample itself all the way through separation and analysis.  Let us help you boost your throughput and improve your workflows with our portfolio of Sample Preparation and LC products that are sure to fit your needs.

  • Tackle challenging matrices with our portfolio of clinical sample preparation products
  • Achieve the sensitivity you need with our core-shell LC products
  • Reliably run your methods, day after day, with reproducible LC and Sample Preparation medias

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How do you pretreat a whole blood sample for SPE?

A simple pretreatment is to add 10% TCA (trichloroacetic acid) aqueous solution in the ratio of 1:1.

Centrifuge and collect the supernatant.  Another simple way to pretreat whole blood is to perform protein precipitation with cold acetonitrile-methanol (85:15) in the ratio of 1:2.  After centrifuging, the supernatant is diluted with solvent appropriate for extraction loading.

A more thorough pretreatment is converting whole blood into blood plasma.  Centrifuging anti-coagulated blood gives blood plasma as supernatant.  Blood plasma preparation kits with prepackaged tubes containing an anticoagulant are commercially available.

For more information on sample pretreatment for extraction using Strata and Strata-X, please contact us.

I have seen you have a number of different applications for Vitamin D, which ones should I consider?

When attempting to analyze vitamin D, it is important to consider the source of the sample.

That is, if samples are from a biological source the vitamin D is likely to be present as the 25-hydroxyvitamin D3 . The difficulty will be in separation of 25-hydroxy and 3-epi-25-hydroxy vitamin D3, and we have applications specific for this critical pair.If the separation is vitamin D from a food stuff or supplement, it will be present just as Vitamin D3 (cholecalciferol) and this is a much more straightforward separation. We have applications on this as well as other fat-soluble vitamins.