HPLC Knowledge Center

What is HPLC?

High Performance Liquid Chromatography (HPLC), also known as High Pressure Liquid Chromatography, is a critical analytical technique extensively used in chemistry and pharmaceuticals for separating, identifying, and quantifying compounds in mixtures. At Phenomenex, we offer specialized HPLC products and solutions to assist scientists in achieving precise and dependable results. Our selection includes columns, consumables, and instruments designed to cater to the diverse requirements of analytical laboratories.

/knowledge-center-hplc-items/query-index.json

tabname

Expert Articles

12

enable

link icon

Fundamental Tips

The importance of a well-prepared mobile phase article

The effects of gradient on buffer capacity article

How can capacity factors drive method robustness? article

The role of end-capping in reversed-phase article

Overcoming peak tailing of basic analytes in silica type a stationary phases in RP article

Basic analytes and the benefits of modified silica supports in reversed-phase LC article

Basic analytes and the use of high pH stable stationary phases article

Scaling Analytical Methods to Prep Chromatography - Part 1 article

Scaling Analytical Methods to Prep Chromatography - Part 2 article

VIDEO My column looks like it is shipped in hexane, but I need to use it in water and acetonitrile. How do I switch the solvents in the column? video

VIDEO How Can I Extend the Lifetime of My SEC Column? video

NEW: VIDEO HILIC explained in one minute video

Method Development Tips

A systematic approach to chiral screening and method development article

Advantages of using immobilized stationary phases in chiral separations article

A systematic approach to peptide analysis article

Method development strategies for oligonucleotides article

The Use of Bio-Inert Hardware in the Analysis of Oligonucleotides article

VIDEO How to Select the Right C18 Chemistry for My Peptide Mapping Analysis? video

VIDEO How to select the right SEC column for the analysis of biologics video

Troubleshooting Tips

Column contamination – what is happening, and how it can be avoided

The danger of mobile phase mismatch

LC System Optimisation for UHPLC Performance on any HPLC

VIDEO: I’m having problems with my peak’s retention time moving but don’t know where to start troubleshooting video

full

true

true

true

tabname

Technical Tips

Also Explore

Knowledge Center Home

Application Library

Blog: Science Unfiltered

Frequently Asked Questions