Product Information
Phase Detail
Biozen Size Exclusion Chromatography (dSEC) Columns

Biozen Size Exclusion Chromatography (dSEC) Columns

Advanced SEC Silica Particle Technology and Surface Chemistry for Characterizing Biomolecules

The Biozen dSEC columns are packed with low pore volume silica coupled with a proprietary hydrophilic diol-type bonded surface chemistry that prevents the silica surface from interacting with protein samples.

  • Exceptionally Robust, Pore Controlled SEC Particle
  • Extreme Stability and Exceptional Lifetime
  • Reproducible Separations

Particle Size
1.8, 3
Recommended Use
Analysis of monoclonal antibodies, biosimilars and other biomolecules by using specific molecular weights with improved column lifetimes and recovery

Biozen SEC Proprietary Silica Particle Technology

The Biozen dSEC columns are packed with low pore volume silica coupled with a proprietary hydrophilic diol-type bonded surface chemistry that prevents the silica surface from interacting with protein samples.

 

Biozen dSEC

Biozen SEC Hydrophilic Surface Chemistry Improves Aggregate Analysis

Improved Aggregate Analysis

Improved Column Lifetime and Performance Stability

Phenomenex’s optimized SEC column loading technology has significantly improved the overall packing density and silica distribution of the columns leading to improved chromatographic lifetime and stability.

Unchanged Performance After 300 Injections

App ID: 26623

Unchanged Performance After 100 Hours of Extreme Running Conditions

New Standard for Platform SEC Methods

Whether IgG2 or IgG4 isotypes, bispecifics, or Fc-Fusions, dSEC-2 provides excellent separation and sample recovery for many different classes of antibodies and related recombinant proteins.

Conditions for both separations:

Column:
Biozen 1.8 µm dSEC-2, 200  Å
Dimension:
300 x 4.6 mm
Part No:
00H-4787-E0
Mobile Phase:
200 Potassium Phosphate + 250 mM KCl, pH 6.2
Flow Rate:
0.35 mL/min
Flow Rate:
0.35 mL/min
Injection Volume:
10 µL
Temperature:
25 ˚C
Detection:
UV @ 280 nm
Sample:
As noted