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biozen-dsec-2

Biozen dSEC-2

Hydrophilic Diol-Bonded SEC Chemistry for Protein and Biotherapeutic Characterization

As part of the Biozen™ dSEC family, Biozen dSEC-2 columns combine controlled pore silica particles with a proprietary hydrophilic diol-bonded surface chemistry designed to minimize secondary interactions and enable reliable size-based separations of proteins and biomolecules.

  • Optimized Pore Structure for Protein Separations
  • Exceptional Stability and Column Lifetime
  • Reproducible SEC Performance Batch After Batch
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Hydrophilic Diol-Bonded SEC Chemistry for Protein and Biotherapeutic Characterization

As part of the Biozen™ dSEC family, Biozen dSEC-2 columns combine controlled pore silica particles with a proprietary hydrophilic diol-bonded surface chemistry designed to minimize secondary interactions and enable reliable size-based separations of proteins and biomolecules.

  • Optimized Pore Structure for Protein Separations
  • Exceptional Stability and Column Lifetime
  • Reproducible SEC Performance Batch After Batch
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Explore Biozen LC Portfolio

With a new titanium BioTi™ biocompatible hardware to minimize priming, four particle platforms for optimal versatility and nine particle chemistries to maximize selectivity and sensitivity, Biozen UHPLC/HPLC columns for protein analysis are seamlessly designed to bring peace of mind to your analysis of biologics through:
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Explore Biozen LC Portfolio

With a new titanium BioTi™ biocompatible hardware to minimize priming, four particle platforms for optimal versatility and nine particle chemistries to maximize selectivity and sensitivity, Biozen UHPLC/HPLC columns for protein analysis are seamlessly designed to bring peace of mind to your analysis of biologics through:
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Overview

Biozen SEC Proprietary Silica Particle Technology

The Biozen dSEC columns are packed with low pore volume silica coupled with a proprietary hydrophilic diol-type bonded surface chemistry that prevents the silica surface from interacting with protein samples.

Biozen SEC Hydrophilic Surface Chemistry Improves Aggregate Analysis

Improved Column Lifetime and Performance Stability

Phenomenex’s optimized SEC column loading technology has significantly improved the overall packing density and silica distribution of the columns leading to improved chromatographic lifetime and stability.

Unchanged Performance After 300 Injections

Unchanged Performance After 100 Hours of Extreme Running Conditions

New Standard for Platform SEC Methods

Whether IgG2 or IgG4 isotypes, bispecifics, or Fc-Fusions, dSEC-2 provides excellent separation and sample recovery for many different classes of antibodies and related recombinant proteins.

Conditions for both separations:

Column:
Biozen 1.8 µm dSEC-2, 200  Å
Dimension:
300 x 4.6 mm
Flow Rate:
0.35 mL/min
Temperature:
25 ˚C
Detection:
UV @ 280 nm
Mobile Phase:
200 Potassium Phosphate + 250 mM KCl, pH 6.2
Part No:
00H-4787-E0
Injection Volume:
10 µL

Sample:
As noted