Native RP-1, developed in tandem with Native RP-5, is an advanced chromatography column designed to revolutionize ADC DAR analysis through orthogonal reversed-phase separation under native conditions, facilitating direct MS detection. While traditional HIC methods offer reproducibility, they are hindered by long method times and high salt mobile phases that are incompatible with MS detection. Native reversed-phase analysis overcomes these challenges, providing comprehensive information that is essential for the development and consistent therapeutic performance of ADC therapeutics.
Separation Mode Reversed Phase |
Recommended Use Non-porous, hydrophobic particle designed to preserve the native forms of intact biomolecules under reverse phase conditions. Suitable for resolving intact DAR and isomer characterization of low to moderate hydrophobic ADCs. |