For security purposes
FOR SECURITY PURPOSES - because Internet Explorer is no longer supported by Microsoft, we suggest that you interact with our secure site through one of our supported browsers - Google Chrome, Firefox, or MS Edge. If you continue to use this website with Internet Explorer you do so at your own risk and you may encounter problems.
Product Information
Phase Detail
Biozen Oligo<br class="t-last-br" />
Biozen Oligo HPLC column provides targeted selectivity for oligos

Biozen Oligo

Advanced Oligonucleotide Analysis

Biozen Oligo is an organo-silica, core-shell particle bonded with a C18 stationary phase. This column offers a unique combination of Core-Shell performance gains such as speed, sensitivity and resolution plus the high pH ruggedness necessary for oligonucleotide separations. Additionally, Biozen Oligo is packaged in BioTi™, a novel bio-inert hardware that serves to alleviate the sample loss and adsorption issues.

Increased Recovery and Reproducibility with NEW Biozen Oligo

  • BioTi™ Hardware Reduces Sample Loss and Adsorption
  • Robustness at High pH and Temperature
  • Core-Shell Advantage for High Efficiency

Separation Mode
Reversed Phase
Pore Size
Particle Size
1.7, 2.6
Recommended Use
Analytical characterization of oligonucleotides

Patented Technology and Advanced Core-Shell Particle Chemistry

Biozen Oligo utilizes organo-silica core-shell technology to:​

  • Minimize band broadening associated with diffusion and oligonucleotide mass transfer kinetics​
  • Increase efficiency to produce narrower peaks, ideal for resolving closely eluting impurities​
  • Patented grafting process ensures high pH and temperature resistance from ethylene cross-linking.​


BioTi versus Traditional Stainless Steel Hardware

LC-MS Analysis of Nusinersen

Oligos can chelate to trace heavy metals in stainless steel column hardware, leading to poor recovery, inconsistent chromatography and problematic carryover. The Biozen Oligo bio-inert hardware provides greater sensitivity as well as improved recovery, demonstrating this column’s optimal utility for oligonucleotide characterization and quantitation.


BioTi vs Stainless Steel Column Hardware​

Biozen Oligo's bioinert hardware enhances recovery, sensitivity, and chromatographic reproducibility by mitigating non-specific adsorption. Stainless steel equivalents fails to prevent this resulting in poor recovery, peak tailing, and residual carryover.​