Biozen Oligo is an organo-silica, core-shell particle bonded with a C18 stationary phase. This column offers a unique combination of Core-Shell performance gains such as speed, sensitivity and resolution plus the high pH ruggedness necessary for oligonucleotide separations. Additionally, bioZen Oligo is packaged in BioTi™, a novel bio-inert hardware that serves to alleviate the sample loss and adsorption issues.
Increased Recovery and Reproducibility with NEW Biozen Oligo
Analytical characterization of oligonucleotides
The Biozen Oligo Columns utilize organo-silica core-shell particles with a highly consistent morphology that minimizes band broadening associated with diffusion and mass transfer, leading to higher efficiency and minimal peak widths, which is critical for the separation of closely eluting impurities associated to synthetic oligonucleotides.
Biozen Oligo uses a patented organo-silica grafting process that incorporates uniform stabilizing ethylene cross-linking to provide resistance to high pH and temperature which are fundamental to reversed phase analysis of oligonucleotides.
Oligos can chelate to trace heavy metals in stainless steel column hardware, leading to poor recovery, inconsistent chromatography and problematic carryover. The bioZen Oligo bio-inert hardware provides greater sensitivity as well as improved recovery, demonstrating this column’s optimal utility for oligonucleotide characterization and quantitation.