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Product Information
Phase Detail
Biozen SEC-2<br class="t-last-br" />
Biozen SEC-2 GFC/SEC columns provide a separation range of 1 k – 450 kDa

Biozen SEC-2

Biozen SEC columns are extremely inert, high density fully porous particle with high efficiency and low molecular weight (LMW) separation range of 1 k – 450 kDa.



Separation Mode
Gel Filtration Chromatography (GFC)
Particle Size
1.8
Recommended Use
Low molecular weight (LMW) separation range of 1 k – 450 kDa

Inside bioZen BioTi™ Hardware

Conditions for both columns:

Columns:
Dimension:
150 x 4.6 mm
Mobile Phase:
50 mM Dipotassium Phosphate + 100 mM Sodium Sulfate, pH 5.0
Flow Rate:
0.3 mL/min
Temperature:
Ambient
Detection:
UV @ 280 nm
Sample:
1. γ-Globulin, 5 mg/mL
2. Ovalbumin, 1 mg/mL

Conditions for both separations:

Column:
bioZen 1.8 μm SEC-2
Dimension:
300 x 4.6 mm
Part No.:
00H-4769-E0
Mobile Phase:
50 mM KH2PO4, 250 mM KCl, pH 6.8
Flow Rate:
0.4 mL/min
Temperature:
30 °C
Detection:
FID @ 280 °C
Samples:
As indicated