Product Information
Phase Detail
Biozen SEC-3<br class="t-last-br" />
Biozen Intact XB-C8 core-shell UHPLC columns provide extremely for impurity profiling and characterization of intact biologic fragments
Biozen SEC-3
Biozen SEC-3
High inertness and particle consistency of both Biozen™ SEC columns drives greater reproducibility from injection-to-injection. Combine this with the bioinert BioTi hardware and good aggregate recovery is no longer something that you’re missing out on.
Biozen SEC columns provide:
• High efficiency on both HPLC and UHPLC
• Extreme inertness and robustness
• Finely tuned high and low MS separation windows
Separation Mode Gel Filtration Chromatography (GFC) |
Pore Size 300 |
Particle Size 1.8 |
Recommended Use High molecular weight (HMW) separation range of 10 k – 700 kDa |
Inside Biozen BioTi Hardware
Conditions for both columns:
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Columns:
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Dimension:
150 x 4.6 mm
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Mobile Phase:
50 mM Dipotassium Phosphate + 100 mM
Sodium Sulfate, pH 5.0
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Flow Rate:
0.3 mL/min
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Temperature:
Ambient
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Detection:
UV @ 280 nm
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Sample:
1. γ-Globulin, 5 mg/mL
2. Ovalbumin, 1 mg/mL |
Aggregate Analysis by Size Exclusion
Conditions same for all samples:
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Column:
Biozen 1.8 µm SEC-3
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Dimension:
300 x 4.6 mm
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Part No.:
00H-4772-E0
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Mobile Phase:
50 mM Potassium Phosphate +
250 mM Potassium Chloride (pH 6.8) |
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Flow Rate:
3.5 mL/min
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Temperature:
30 °C
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Detection:
UV @ 280 nm
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Sample:
As noted
|
Conditions same for all samples:
|
Column:
Biozen1.8 µm SEC-3
|
|
|
Dimension:
300 x 4.6 mm
|
|
|
Part No.:
00H-4772-E0
|
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Mobile Phase:
50 mM Potassium Phosphate +
250 mM Potassium Chloride (pH 6.8) |
|
|
Flow Rate:
3.5 mL/min
|
|
|
Temperature:
30 °C
|
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Detection:
UV @ 280 nm
|
|
|
Sample:
As noted
|
Conditions same for all samples:
|
Column:
Biozen 1.8 µm SEC-3
|
|
|
Dimension:
300 x 4.6 mm
|
|
|
Part No.:
00H-4772-E0
|
|
|
Mobile Phase:
50 mM Potassium Phosphate +
250 mM Potassium Chloride (pH 6.8) |
|
|
Flow Rate:
3.5 mL/min
|
|
|
Temperature:
30 °C
|
|
|
Detection:
UV @ 280 nm
|
|
|
Sample:
As noted
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