HPLC
22
3032
biozenlc-hplc-column
biozen-sec-3
Biozen SEC-3
High inertness and particle consistency of both Biozen™ SEC columns drives greater reproducibility from injection-to-injection. Combine this with the bioinert BioTi hardware and good aggregate recovery is no longer something that you’re missing out on.
Biozen SEC columns provide:
• High efficiency on both HPLC and UHPLC
• Extreme inertness and robustness
• Finely tuned high and low MS separation windows
Biozen SEC columns provide:
• High efficiency on both HPLC and UHPLC
• Extreme inertness and robustness
• Finely tuned high and low MS separation windows
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Overview
Inside Biozen BioTi Hardware
Conditions for both columns:
Column:
Dimension
150 x 4.6 mm
Flow Rate:
0.3 mL/min
Temperature:
Ambient
Detection
UV @ 280 nm
Mobile Phase:
50 mM Dipotassium Phosphate + 100 mM Sodium Sulfate, pH 5.0
Sample:
- γ-Globulin, 5 mg/mL
- Ovalbumin, 1 mg/mL
Aggregate Analysis by Size Exclusion
Conditions same for all samples:
Column:
Biozen 1.8 µm SEC-3
Dimension:
300 x 4.6 mm
Flow Rate:
3.5 mL/min
Temperature:
30 °C
Detection
UV @ 280 nm
Mobile Phase:
50 mM Potassium Phosphate +
250 mM Potassium Chloride (pH 6.8)
250 mM Potassium Chloride (pH 6.8)
Part No.:
00H-4772-E0
Sample:
As noted
Conditions same for all samples:
Column:
Biozen 1.8 µm SEC-3
Dimension
300 x 4.6 mm
Flow Rate:
3.5 mL/min
Temperature:
30 °C
Detection
UV @ 280 nm
Mobile Phase:
50 mM Potassium Phosphate +
250 mM Potassium Chloride (pH 6.8)
250 mM Potassium Chloride (pH 6.8)
Part No.:
00H-4772-E0
Sample:
As noted
Conditions same for all samples:
Column:
Biozen 1.8 µm SEC-3
Dimension:
300 x 4.6 mm
Flow Rate:
3.5 mL/min
Temperature:
30 °C
Detection:
UV @ 280 nm
Mobile Phase:
50 mM Potassium Phosphate +
250 mM Potassium Chloride (pH 6.8)
250 mM Potassium Chloride (pH 6.8)
Part No.:
00H-4772-E0
Sample:
As noted
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