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biozenspe-sample-preparation-products

Biozen Sample Preparation Products

MagBeads

Streptavidin coated paramagnetic particle, ideal for isolating proteins and peptides in less time with a high response. With a unique magnetic bead particle and rigorous specifications, Biozen MagBeads are created to ensure uniform beads that provide consistent and reliable results. Pairs with: Biozen XB-Peptide and Peptide LC Columns

N-Glycan Clean-Up SPE

Solid phase extraction (SPE) HILIC stationary phase that excels at retention and recovery labeled released N-glycans! Microelution format allows for streamlined processing and clean-up of small sample volumes.

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Overview

Peptide MagBeads

Streptavidin Coated

Uniform magnetic particles result in faster and reliable purification, clean-up, and isolation of proteins and peptide molecules.

MagBead Activation

Immunocapture

Glycan: N-Glycan Clean-Up

Streptavidin Coated
Uniform magnetic particles result in faster and reliable purification, clean-up, and isolation of proteins and peptide molecules.

STEP 1

Start with Intact Glycoprotein

STEP 2

Deglycosylation Released N-Glycans by PNGase F in buffer solution

STEP 3

Labeling Glycans 2-AA or 2-AB for accurate and sensitive glycan detection

STEP 4

Clean-Up Removal of labeling reagent, salts, or surfactants

STEP 5

Analysis

96-Well Plate:
Biozen N-Glycan Clean-up
Part No.:
8M-S009-NGA
Condition:
200 µL Water
Equilibrate:
200 µL Water/Acetonitrile (15:85)
Load:
Labeled Sample (up to 400 µL of labeled sample)
Wash:
2x 600 µL Formic acid/Water/Acetonitrile (1:9:90)
Elute:
3x 30 µL 200 mM Ammonium acetate in Acetonitrile/Water (5:95) of labeled sample)
Dilute:
100 µL Dimethylformamide and then 210 µL of Acetonitrile
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Applications

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