Clarity Oligo-MS C18 columns have been engineered for the MS characterization of synthetic DNA and RNA samples. This media is based on core-shell technology which generates extremely high efficiencies due to the innovative particle design. This increase in efficiency improves the resolution between critical oligo sequences, gives higher sensitivity for easier MS quantitation, and allows for a decrease in column length for higher throughput.
Particle Size 1.7, 2.6 |
Recommended Use Rapid LC-MS analysis and QC of synthetic oligonucleotide research reagents and therapeutics |
Column:
Clarity 2.6 µm Oligo-MS C18
|
Dimension:
50 x 2.1 mm
|
Part No.:
00B-4479-AN
|
Mobile Phase:
A: 100 mM HFIP / 4 mM TEA / 2 % Methanol
B: 100 mM HFIP / 4 mM TEA / 98 % Methanol |
Gradient:
A/B (95:5) to A/B (80:20) in 10 min
|
Flow Rate:
0.5 mL/ min
|
Temperature:
50 ˚C
|
Detection:
UV @ 260 nm (22 ˚C)
|
Injection Volume:
20 µL
|
Sample Volume:
Poly dT (12-18)
|
Column:
Clarity 2.6 µm Oligo-MS C18
|
Dimension:
50 x 2.1 mm
|
Part No.:
00B-4479-AN
|
Mobile Phase:
A: 100 mM HFIP / 4 mM TEA / 2 % Methanol
B: 100 mM HFIP / 4 mM TEA / 98 % Methanol |
Gradient:
A/B (95:5) to A/B (80:20) in 10 min
|
Flow Rate:
0.5 mL/ min
|
Temperature:
50 ˚C
|
Detection:
UV @ 260 nm (22 ˚C)
|
Injection Volume:
20 µL
|
Sample Volume:
Crude DNA 30mer
|