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clarity-qsp
Clarity QSP
High-Throughput Purification
Purify trityl on synthetic DNA or RNA samples in 20 minutes using Clarity QSP 96-well plate and buffer system. Final elution delivers >90 % typical purities/recoveries for in vivo applications and downstream analysis by LC-MS or CE.
- Formats offer easy high-throughput processing for parallel purifications
- Simple three step process delivers highly purified DNA/RNA in minutes
- For oligos 10-100 mer
- Economical and disposable format
U.S. Patent No. 7,119,145
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General Extraction
Formats
Oligo Chemistry
96-Well Plates and 3 mL Cartridges
Synthesis Scale Load
DNA & RNA
Oligo Length
50 mg/well 96-well plates and 60 mg/3 mL tubes=up to 200 nmol.
150 mg/ 3 ml tubes= up to 1 µmol.
5 g/60 mL tubes= up to 25 µmol.
Typical Purity1
≤ 100 nt
Typical Recovery Yield2
≥ 90 %
Typical Recovery Yield2
≥ 80 %
Purification Time
~ 8 minutes/ cartridge, ~ 45 minutes/ well plate (96 samples)
Equipment Required
Positive pressure manifold, vacuum manifold, or automated liquid handling system
1 Purity value based on ion-exchange chromatography and capillary electrophoresis
2 OD260 used for quantitation
It’s Quick, Simple, and Pure (QSP)
Pre-treatment
Trityl-on oligo sample preparation. Mix equal volume of loading buffer with cleavage/deprotection solution
Clarity QSP 96-well plate with DNA Buffer
Crude 30 mer DNA- 200 nmole Sequence: GTGGATCTGCGCACTTCAGGCTCCTGGGCG
Sample Preparation:
- 250 µL of oligo solution in concentrated NH2OH (post deprotection) was aliquoted
- An equal volume of DNA buffer (250 µL) was added and vortexed
Condition:
1 mL Methanol (2 x 0.5 mL)
Equilibrate:
1 mL Water (2 x 0.5 mL
Load:
Oligo Sample (500 µL)
Detritylate:
1 mL 1 % DCA
Rinse:
1 mL Water (2 x 0.5 mL)
Dry Sorbent:
at 10” Hg Vacuum (~1 min)
Elute:
1 mL 15 mM Na2CO3/ 20 % Acetonitrile
Crude Trityl-on
Load Fraction
Detritylated Final Elution
Recovery
Purity (Peak area)
28.3
5.3
20.8
90.3 %
92 %
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