Di-isobutyl side chains differentiate this C18 column. Low ligand density and an inactive surface make this column a great hydrogen bond acceptor. This phase will demonstrate improved peak shape and increased retention of acidic compounds.
*Kinetex analytical columns are compatible with all LC system with standard LC column end-fittings threaded in 10-32. Please contact us at Phenomenex.com/chat for any questions and information about fitting compatibility and any other column internal diameters.
Carbon Load 10 (effective), 10.0, null %, 10 (effective) %, 10.0 % |
Surface Area (m²/g) 200 |
Particle Size 1.7, 2.6, 3.5, 5 |
Recommended Use Superior peak shape and enhanced separation of basic compounds under neutral and acidic conditions |
This unique C18 phase yields increased hydrogen bonding with hydrophobic selectivity‚ resulting in improved peak shape for basic compounds and increased retention of acidic compounds.
Dimension:
50 x 2.1 mm
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Mobile Phase:
A: Water B: Acetonitrile |
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Gradient:
Time (min)
0
6 6.01 8 | % B
20 60 20 20 |
Flow Rate:
0.5 mL/min |
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Temperature:
30 °C |
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Detection:
UV @ 220 nm (ambient) |
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Samples:
1. Estriol 2. Hydrocortisone 3. Cortisone 4. Estradiol 5. Cortisone-21-acetate 6. 21-Hydroxyprogesterone 7. Estrone 8. 17-Hydroxyprogesterone 9. Deoxycorticosterone acetate |
Dimension:
50 x 2.1 mm
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Mobile Phase:
A: Water with 0.1% Formic Acid B: Acetonitrile with 0.1% Formic Acid |
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Gradient:
Time (min)
0
0.2 4.2 4.21 5.5 | % B
5 5 95 5 5 |
Flow Rate:
0.8 mL/min |
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Temperature:
30 °C |
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Detection:
UV @ 245 nm (ambient) |
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Samples:
1. Pyridine 2. Acetaminophen 3. Pindolol 4. Quinidine 5. Sulfathiazole 6. Acebutolol 7. Benzyl alcohol 8. Chlorpheniramine 9. Phenol 10. Triprolidine 11. Nortriptyline 12. Prednisolone 13. 3-Methyl, 4-nitrobenzoic acid 14. 2-Hydroxy, 5-methylbenzaldehyde 15. Diflunisal 16. Hexanophenone |