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Product Information
Phase Detail
Luna Phenyl-Hexyl HPLC Columns
Luna Phenyl-Hexyl HPLC columns is a hydrophobic phenyl phase that can be used for L11 USP methods

Luna Phenyl-Hexyl HPLC Columns

The Luna brand of columns and media is more than just a product line from Phenomenex. It is a pledge to provide you with the highest level of satisfaction for your chromatographic goals. The Luna Phenyl Hexyl is our most hydrophobic phenyl column and it will also provide good hydrogen accepting functionality for acidic retention.

  • Extensive line of rugged particle sizes
  • Easy method scalability from HPLC to preparative and bulk purification LC
  • Phenyl-Hexyl Ultra-pure, metal-free silica (99.99% purity) (USP: L11)

Carbon Load
Surface Area (m²/g)
Particle Size
3, 5, 10, 15
Recommended Use
Separation of aromatic and closely related compounds

Long Column Lifetimes and Excellent Performance

Ultra-pure, metal-free silica (99.99 % purity) is the backbone of all Luna material. The resulting high quality particles have a surface smoothness, pore structure, and pore consistency to ensure a more uniform particle shape and greater reproducibility.

Superior Particle Smoothness

Waters® Symmetry®
Agilent Technologies® ZORBAX® 5 µm SB-C18
Agilent Technologies® ZORBAX®
Waters® Symmetry® 5 µm C18
Phenomenex Luna
Phenomenex Luna 5 µm C18

Engineered for Stability

Luna Phenyl-Hexyl columns provide separations not achievable on C18 or C8 columns; such as increased retention for polar‚ aromatic compounds as well as reversals in analyte elution order. Luna Phenyl-Hexyl columns are a reproducible‚ extremely stable phenyl phase.

Comparison of Phenyl Columns: Antibacterial

Comparison of Phenyl Columns: Antibacterial
Conditions for all columns:

150 x 4.6  mm
Mobile Phase:
A: 20 mM KH2PO4, pH 2.5
B: Acetonitrile
A/B (80:20) to A/B (75:25) in 5 min, then to A/B (55:45) in 15 min
Flow Rate:
1.0 mL/min
22 °C
UV @ 254 nm
1. Carbadox
2. Thiamphenicol
3. Furazolidone
4. Oxolinic acid
5. Sulfadimethoxine
6. Sulfaquinoxaline
7. Nalidixic acid
8. Piromidic acid

Combined Selectivity Characteristics

Dual selectivity of both phenyl phase and a short alkyl phase

Cough and Cold Medicine

Conditions for all columns:

Luna 3 µm Phenyl-Hexyl
75 x 4.6  mm
Part No.:
Mobile Phase:
A: Acetonitrile
B: 20 mM KH2PO4 / Methanol (80:20) pH 9.0
A/B (0:100) to A/B (80:20) in 5 min
Flow Rate:
1.0 mL/min
20 µL
22 °C
UV @ 214 nm
1. p-Aminophenol
2. Benzoic acid
3. Acetaminophen
4. Pseudoephedrine
5. Butyl paraben
6. Chlorphenaramine
7. Diphenhydramine
8. Dextromethorphan