Improved recovery of basic drugs from biologic samples in a funnel shaped tube ideal for large sample volumes with minimal extraction solvent. Small bed mass requires only minimal extraction solvents for more concentrated extracts by incorporating the hydrophobic selectivity of a C8 phase and strong cation-exchange.
Condition
1 mL Methanol
|
Equilibrate
1 mL Water |
Load
Pretreated sample |
Wash
1 mL 25 mM Ammonium Acetate Buffer, pH 6-7 |
Wash 2
1 mL Methanol, dry under vacuum for 2-5 min |
Elute
1 mL 5 % Formic Acid in Methanol |
Based on 100 mg/1 mL sorbent mass. The above is a convenient starting point for SPE
method development, further optimization may be required to tailor the method for your
specific extraction needs. |