A reversed phase functionalized polymeric sorbent that gives strong retention of neutral, acidic, or basic compounds under aggressive, high organic wash conditions. This sorbent relies on 3 mechanisms of retention: pi-pi bonding, hydrogen bonding (dipole-dipole interactions), and hydrophobic interaction.
Brand Strata-X |
Recommended Use Extraction of basic drugs from biological samples |
Ï€-Ï€ Bonding
Hydrogen Bonding
Dipole-Dipole Interactions
Hydrophobic Interaction
Column:
1 mL Methanol
|
Equilibrate:
1 mL Water
|
Load:
Diluted Sample
|
Wash:
1 mL 5-60 % Methanol
|
Elute:
2x 500 µL 2 % Formic Acid in Methanol/Acetonitrile
|
Based on 30 mg/1 mL sorbent mass. The above is a convenient starting point for SPE method development, further optimization may be required to tailor the method for your specific extraction needs.