Strata-X-A SPE Products

Strong Anion Mixed-Mode Solid Phase Extraction (SPE)

A strong anion-exchange functionalized polymeric sorbent that allows for complete retention of weakly acidic compounds with a pK_a greater than 2, making 100% organic wash conditions possible. A 100% organic wash ensures that the maximum amount of interferences are removed from the target compound.

Brand Strata-X
Recommended Use Extraction of basic drugs from biological samples

How It Works
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3 Mechanism of Retention

Strong Anion-Exchange

π-π Bonding

Hydrophobic Interaction

Available Formats

96-Well Plates

Microelution 96-Well Plates

1, 3, and 6 mL Tubes

Giga™ Tubes (12-150 mL Tubes)

Starting Method

For Acids with pK_a > 2

Column: 1 mL Methanol
Equilibrate: 1 mL Water, pH 6-7
Load: Diluted Sample pH 6-7
Wash 1: 1 mL 25 mM Ammonium Acetate Buffered, pH 6-7
Wash 2: 1 mL Methanol (collect this fraction to analyze Neutral/Bases)
Elute Acid: 2x 500 µL 5 % Formic Acid in Methanol

Based on 30 mg/1 mL sorbent mass. The above is a convenient starting point for SPE method development, further optimization may be required to tailor the method for your specific extraction needs.