Strata-X-CW SPE Products
Weak Cation Mixed-Mode Solid Phase Extraction (SPE)
A weak cation-exchange functionalized polymeric sorbent that allows for complete retention of basic compounds with a pKa greater than 8, making 100% organic wash conditions possible. A 100% organic wash ensures that the maximum amount of interferences are removed from the target compound.
SP
292
2135
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Overview
3 Mechanism of Retention
4
Weak Cation-Exchange
false
π-π Bonding
false
Hydrophobic Interaction
false
Available Formats
4
96-Well-Plate
false
96-Well-Plate
false
1, 3, and 6mL Tubes
false
Giga Tubes (12-150mL Tubes)
false
On-line Cartridges
false
Starting Method
For Bases with pKa > 8
Column
1mL Methanol
Equilibrate
1 mL Water, pH 6-7
Load
Diluted Sample, pH 6-7
Wash 1
1 mL Water, pH 6-7
Wash 2
1 mL Methanol (collect this fraction to analyze Neutrals/Acids)
Elute Bases
2x 500 µL 5 % Formic Acid in Methanol
Elute Weak Acids
2x 500 µL 5 % NH4OH in Methanol
Based on 30 mg/1 mL sorbent mass. The above is a convenient starting point for SPE method development, further optimization may be required to tailor the method for your specific extraction needs.
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