A strong cation-exchange functionalized polymeric sorbent that allows for complete retention of basic compounds with a pKa less than 10.5, making 100% organic wash conditions possible. A 100% organic wash ensures that the maximum amount of interferences are removed from the target compound. The large particle format is ideal for processing large volumes or viscous samples.
Brand Strata-X |
Recommended Use Extraction of weakly basic compounds, present in large volume or viscous samples |
Strong Cation-Exchange
π-π Bonding
Hydrophobic Interaction
96-Well-Plate
1, 3, and 6mL Tubes
Giga Tubes (12-150mL Tubes)
Column:
1 mL Methanol
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Equilibrate:
1 mL Acidified Water
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Load:
Diluted Acidified Sample
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Wash 1:
1 mL 0.1 N HCI in Water (collect this fraction to analyze Polar Neutrals)
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Wash 2:
1 mL 0.1 N HCI in Methanol (collect this fraction to analyze Neutrals/Acids)
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Elute Any Acids:
2x 500 µL 5 % NH4OH in Methanol
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Based on 30 mg/1 mL sorbent mass. The above is a convenient starting point for SPE method development, further optimization may be required to tailor the method for your specific extraction needs.