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Zebron ZB-FAME GC Columns

The Fast FAME GC Column

  • Reduce run times for traditional FAMEs separations up to 75 %
  • Separate cis/trans FAME isomers with Rs ≥ 1.0
  • High-cyanopropyl selectivity acceptable for use with AOCS, AOAC, and IOC methods

Application Notes:

  • Reproducible FAME Analysis on a 100 meter Zebron™ ZB-FAME GC Column. Read more.
  • Demonstration of Robustness and Selectivity for the Analysis of FAMEs in Salmon Oil and Pet Food. Read more.

Recommended Use
Fatty acid methyl esters (FAMEs), cis/trans FAME isomers, Omega 3 and Omega 6 FAMES

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Overview

Upgrade to Zebron from any high cyanopropyl phase:

Agilent®
Supelco®
CP-Sil 88
SP®-2380
HP-88
SP-2560
DB®-23

Accelerating FAME Testing

Designed to move beyond the conventional to the exceptional , Zebron GC columns come to life through a coupling of innovative spirit and technical excellence. As part of the Zebron Unlimited series of columns, Zebron ZB FAME offers unparalleled performance through the power of targeted selectivity when analyzing fatty acid methyl esters (FAMEs)

Optimized High-Cyano Polarity
FAME separations require the use of high polarity columns for adequate resolution of cis/trans isomers. Zebron™ ZB-FAME is an optimized high-cyanopropyl (G48) chemistry that provides the polarity that you need, while remaining compatible with AOAC,  OCS, and IOC regulatory methods.

Elution Order for 37 FAME Standard

Peak
Compound
ID
1
Butanoic Acid Methyl Ester
C4:0
2
Hexanoic Acid Methyl Ester2
C6:0
3
Octanoic Acid Methyl Ester
C8:0
4
Decanoic Acid Methyl Ester
C10:0
5
Undecanoic Acid Methyl Ester
C11:0
6
Dodecanoic Acid Methyl Ester
C12:0
7
Tridecanoic Acid Methyl Ester
C13:0
8
Myristic Acid Methyl Ester
C14:0
9
Myristoleic Acid Methyl Ester
C14:1 cis 9
10
Pentadecanoic Acid Methyl Ester
C15:0
11
cis-10-Pentadecenoic Acid Methyl Ester
C15:1 cis 10
12
Hexadecanoic Acid Methyl Ester
C16:0
13
Palmitoleic Acid Methyl Ester
C16:1 cis 9
14
Heptadecanoic Acid Methyl Ester
C17:0
15
cis-10-Heptadecenoic Acid Methyl Ester
C17:1 cis 10
16
Stearic Acid Methyl Ester
C18:0
17
Elaidic Acid Methyl Ester
C18:1 trans 9
18
Oleic Acid Methyl Ester
C18:1 cis 9
19
Linolelaidic Acid Methyl Ester
C18:2 trans 9,12
Peak
Compound
ID
1
Linoleic Acid Methyl Ester
C18:2 cis 9,12
2
γ-Linolenic Acid Methyl Ester
C18:3 cis 6,9,12
3
α-Linolenic Acid Methyl Ester
C18:3 cis 9,12,15
4
Arachidic Acid Methyl Ester
C20:0
5
cis-11-Eicosenoic Acid Methyl Ester
C20:1 cis 11
6
cis-11,14-Eicosadienoic Acid Methyl Ester
C20:2 cis 11,14
7
Heneicosanoic Acid Methyl Ester
C21:0
8
cis-8,11,14-Eicosatrienoic Acid Methyl Ester
C20:3 cis 8,11,14
9
Arachidonic Acid Methyl Ester
C20:4 cis 5,8,11,14
10
cis-11,14,17-Eicosatrienoic Acid Methyl Ester
C20:3 cis 11,14,17
11
Behenic Acid Methyl Ester
C22:0
12
Erucic Acid Methyl Ester
C22:1 cis 13
13
cis-5,8,11,14,17-Eicosapentaenoic Acid Methyl Ester
C20:5 cis 5,8,11,14,17
14
cis-13,16-Docosadienoic Acid Methyl Ester
C22:2 cis 13,16
15
Tricosanoic Acid Methyl Ester
C23:0
16
Lignoceric Acid Methyl Ester
C24:0
17
Nervonic Acid Methyl Ester
C24:1 cis 15
18
cis-4,7,10,13,16,19-Docosahexaenoic Acid Methyl Ester
C22:6 cis 4,7,10,13,16,19

QC Tested For FAMEs

Baseline Separation of Common Isomers

Food and dietary standards require separation of cis/trans fatty acids. Zebron™ ZB-FAME is able to separate from C4 to C24 with resolution of specific cis/trans fatty acids.

Completely Resolve AOAC Critical Pairs

Columns:
Zebron ZB-FAME
Dimension:
30 meter x 0.25 mm x 0.20 µm
Detector:
FID @ 260 °C
Part No.:
7HG-G033-10
Injection:
Split 50:1 @ 240 °C, 1 µL
Recommended Liner:
Zebron PLUS Single Taper with Wool
Liner Part No.:
AG2-0A11-05 (for Agilent® systems)
Carrier Gas:
Helium @ 1.2 mL/min (constant flow)
Oven Program:
100 °C for 2 min to 140 °C @ 10 °C/min
to 190 °C @ 3 °C/min to 260 °C @ 30 °C/min for 2 min

Sample:

37 FAME standard

Separate Detailed C18 Compounds

Due to regulatory guidelines around trans fat content in food, detailed analysis of specific FAMEs isomers is commonly performed. The below methods demonstrate this testing using a 60 meter Zebron™ ZB-FAME column.

Analysis of C18:1, C18:2, and C18:3

Columns:
Zebron ZB-FAME
Dimension:
60 meter x 0.25 mm x 0.20 µm
Detector:
FID @ 260 °C
Part No.:
7KG-G033-10
Injection:
Split 50:1 @ 240 °C, 1 µL
Recommended Liner:
Zebron PLUS Single Taper with Wool
Liner Part No.:
AG2-0A11-05 (for Agilent® systems)
Carrier Gas:
Helium @ 30 cm/sec (constant flow)
Oven Program:
160 °C (isothermal)

Sample:

As listed. For full peak identification, visit www.phenomenex.com/DetailedFAME

Twice The Runs, Half The Time

37 FAMEs In A Short 11 Minute Run

Traditionally, cis/trans FAME separations require the use of long (100 meters or more) columns and can run up to 60 minutes, resulting in a bottleneck to higher productivity. Zebron™ ZB-FAME provides targeted selectivity that allows for reduced column length – run times as short as 11 minutes without compromising your results!

Columns:
Zebron ZB-FAME
Dimension:
20 meter x 0.18 mm x 0.15 μm
Detector:
FID @ 260 °C
Part No.:
7FD-G033-05
Injection:
Split 100:1 @ 250 °C, 1 µL
Recommended Linear:
Zebron PLUS Single Taper Z-Liner™
Liner Part No.:
AG2-0A13-05 (for Agilent® systems)
Carrier Gas:
Helium @ 1.0 mL/min (constant flow)
Oven Program:
80 °C for 1.5 min to 160 °C @ 40 °C/min to 185 °C @ 5 °C/min to 260 °C @ 30 °C/min

Sample:

37 FAME standard

23839, 23821, 24077, 23928, 23825

Applications

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