Extraction of Ibuprofen from Serum on Strata™ X

Sample Preparation Details

Part Number :8B-S100-TAK
Description :Strata-X 33 µm Polymeric Reversed Phase, 30 mg / 1 mL, Tubes, 100/Pk
Condition 1 :1.0 mL Methanol
Condition 2 :1.0 mL Water
Load :Load 1.00 mL Sample diluted in Buffer
Wash 1 :1.0 mL 5% methanol/D. I. water
Dry :1 min at 10 '' of Hg
Elution 1 : Methanol
Evaporate: :Evaporate to dryness
Reconstitute :2 mL Mobile Phase
Notes :Blow down to almost dryness under slow stream of N2. Reconstitute with mobile phase to 200 µL. Inject 50 µL on HPLC

Application Detail (App ID: 14454)

Part No:00F-4249-E0
Dimensions:150 x 4.6 mm ID
Elution Type:Isocratic
Elution A:0.05% Phosphoric Acid (pH 2.3)
Elution B:Acetonitrile
Gradient Profile:
Step No. Time (min) Pct A Pct B
1 0 50 50

Flow Rate:1.5 mL/min
Col. Temp: ambient
Detection: UV-Vis Abs.-Fixed Wave. (UV) @ 230 nm (22°C)
Note:SecurityGuard™ Guard Cartridge System extends column lifetime.
- SecurityGuard Cartridges, C8 4 x 3.0mm, 10/Pk Part No.: AJ0-4290
- Holder Part No.: KJ0-4282

Analyte Details

Analyte Detail not available