Protein Calibration Curves on BioSep-SEC-S (2)

Application Detail (App ID: 18927)

Part No:00H-2146-K0
Dimensions:300 x 7.8 mm ID
Elution Type:Isocratic
Elution A:100mM Sodium Phosphate, 300mM NaCl, pH 7.0
Gradient Profile:
Step No. Time (min) Pct A
1 0 100

Flow Rate:1 mL/min
Col. Temp: ambient
Detection: UV-Vis Abs.-Variable Wave.(UV) @ 280 nm (22°C)
Detector Info: 3rd order of polynomial MW calibration curves
Note:Application Focus: To show the calibration curves for the three different BioSep-SEC phases

For this application, calibration curves are generated for the three different BioSep-SEC phases using standard proteins of defined molecular weights and non-denaturing operating conditions (100 mM phosphate, 300 mM NaCl, pH 7.0, 1mL/min). For each media (300 x 7.8 mm column) the elution volume where each protein peak elutes was plotted against the log of the expected molecular weight of each protein. A 3rd order polynomial is used to plot the calibration curve for each phase.

Specific applications used to generate curves were: App ID# 18892 for BioSep2000, App ID# 18928 for BioSep3000 and App ID#18936 for BioSep4000. While a polynomial was used to generate calibration curves in this example, one could use a linear plot if one removes the data points at the ends of the molecular weight ranges.

Analyte Details

Analyte Detail not available

11 Proteins