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A: Buffer (dilute 7 mL of triethylamine with water to 1 L. Stir and adjust with phosphoric acid to a pH of 3.0+/- 0.1), Methanol and Acetonitrile (58:25:17)
Gradient Profile:
Step No.
Time(min)
%A
1
20
100
Flow Rate:
1.4 mL/min
Temperature:
30°C
System:
Agilent Technologies 1100 Series Agilent Technologies 1100 Series