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Product Information
Phase Detail
Luna Omega Polar C18 HPLC Columns
Achieve excellent separation and high sensitivity with Luna Omega Polar C18 HPLC columns. Explore premium chromatography solutions and performance at Phenomenex.
Luna Omega Polar C18 HPLC Columns
Luna Omega Polar C18 HPLC Columns
Reversed Phase Polar Retention Without the Headache
Luna Omega Polar C18 is a novel HPLC and UHPLC stationary phase capable of providing a unique selectivity within a wide elution window and increased retention for both polar and non polar analytes. The all purpose C18 ligand provides hydrophobic interactions while a polar modified particle surface provides enhanced polar retention and also aqueous stability. These attributes make the Luna Omega Polar C18an excellent choice for balanced retention of polar and hydrophobic compounds as well as to solely enhance retention of highly polar compounds.
- Compatible with All Analytical HPLC/UHPLC Systems*
- Fully Scalable Particle Size UHPLC HPLC Preparative
- 100 % Aqueous Stable C18 Selectivity
Scalable Particle Sizes
*Luna Omega analytical columns are compatible with all LC system with standard LC column end fittings threaded in 10-32. Please contact us at Phenomenex.com/chat for any questions and information about fitting compatibility and any other column internal diameters.
Carbon Load 11.0 % |
Particle Size 1.6, 3, 5 |
Recommended Use Polar retentive C18 phase for HPLC and UHPLC separations of hydrophobic/polar compounds |
Not Just Another C18…Built Different
Luna Omega Polar C18 is a uniquely modified C18 based chemistry that has been optimized to improve the performance of polar analyses. This new particle surface chemistry makes the Polar C18 applicable to all industries that utilize HPLC for mixtures of polar and non polar compounds.
Stable Retention & 100% Aqueous Stable
Traditional C18 phases are known to collapse under 100 % aqueous conditions, causing retention loss of compounds and a method development headaches. That is why an advanced proprietary bonding technology was used for the Luna Omega Polar C18 in order to ensure aqueous stability. The graph below displays the excellent stability of Polar C18 in 100% aqueous buffer conditions for over 2 weeks.
Conditions for all columns:
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Columns:
Luna Omega 1.6 µm Polar C18
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Dimension:
50 x 2.1 mm
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Part No.:
00B-4748-AN
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Mobile Phase:
10 mM Ammonium Formate
with 0.1 % Formic Acid |
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Flow Rate:
0.4 mL/min
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Temperature:
22 °C
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Detection:
UV @ 254 nm
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Samples:
1. Adenine
2. Guanosine-5-Monophosphate 3. Thymine |
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Duration of Exposure
 Day 1 Day 25 |
Increased Reversed Phase Polar Retention
With its polar modified surface, the Luna Omega Polar C18 offers UHPLC users enhanced separation power that can greatly improve resolution values for target compounds.
Pharmaceutical Compound Mixture
Columns:
Luna Omega 1.6 µm Polar C18
Kinetex 1.7 µm C18 |
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Dimension:
50 x 2.1 mm
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Mobile Phase:
A: 10 mM Ammonium Formate with 0.1 % Formic Acid B: Acetonitrile with 0.1 % Formic Acid |
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Gradient:
Time (min)
0
3 | % B
0 90 |
Flow Rate:
0.4 mL/min |
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Temperature:
22 °C |
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Injection Volume:
1 µL |
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Detection:
MS/MS (SCIEX API 4000â„¢) |
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Samples:
1. 3-Hydroxyphenol 2. Phenol 3. 4-Nitrophenol 4. 4-Methylphenol 5. 2-Methylphenol 6. 2,4-Dimethylphenol 7. 1-Naphthol |
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Dipeptides in 100 % Aqueous Conditions
Columns:
Luna Omega 1.6 µm Polar C18
Competitor 1.7 µm C18 |
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Dimension:
50 x 2.1 mm
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Part No.:
00B-4748-AN
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Mobile Phase:
A: Water with 0.1 % TFA B: Acetonitrile with 0.1 % TFA |
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Gradient:
Time (min)
0
3 | % B
0 75 |
Flow Rate:
0.6 mL/min |
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Temperature:
40 °C |
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Injection Volume:
1 µL |
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Detection:
UV @ 210 nm (ambient) |
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Samples:
1. Arg-Glu 2. Gly-Tyr 3. Trp-Gly 4. Gly-Trp 5. Pro-Trp |
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Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide.
Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide.