Polar Probes on Synergi Polar

Analytes

1Cytosine
2Adenine
3Thymine
4Morphine
5Hydromorphone

Compound Name Guanine
CID 764
Molecular Formula C5H5N5O
Molecular Weight 151
No. Hydrogen Bond Acceptors 4
No. Hydrogen Bond Donors 3

Details

LC Conditions (App ID: 17944)

Column:

Synergi 4 µm Polar-RP 80 Å, LC Column 150 x 4.6 mm, Ea

Brand Name:

Synergi

Part No:

00F-4336-E0

Phase Name:

Polar-RP

Elution Type:

Gradient

Mobile Phase:

A: Phosphate Buffer, pH 2.5

B: Acetonitrile

C: 0

D: 0

Gradient Profile:

Step No.	Time(min)	%A	%B
1	0	100	0
2	5	100	0
3	5.01	95	5
4	10	70	30
5	10.5	100	0
6	14	100	0

Flow Rate:

1 mL/min

Temperature:

22°C

Detection:

UV-Vis Abs.-Diode Array (PDA) @ 210 nm (22°C)

Sample Note:

SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, Polar-RP 4 x 3.0mm ID, 10/Pk Part No.: AJ0-6076 - Holder Part No.: KJ0-4282

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