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Applications
Alfatoxins in Food by LC/MS/MS
20016
Separation Mode: Reversed Phase
Reversed Phase
PFP(2)
HPLC
Poisons
Food & Beverage
Biochemical Compound / Nutrient
Product / Trade Good
Environmental / Health
Therapeutic / Clinical

Applications

Alfatoxins in Food by LC/MS/MS
Analytes
1Aflatoxin M1
2Aflatoxin G2
3Aflatoxin G1
4Aflatoxin B2
5Aflatoxin B1
Compound Name
Aflatoxin M1
CID
23236
Molecular Formula
C17H12O7
Molecular Weight
328
No. Chiral Centers:
2
No. Hydrogen Bond Acceptors
7
No. Hydrogen Bond Donors
1
Details
LC Conditions (App ID: 20016)
Column:
Brand Name:
Part No:
Phase Name:
PFP(2)
Elution Type:
Isocratic
Mobile Phase:
A: 5mM Ammonium Acetate + 0.1% Formic Acid
B: Methanol
Gradient Profile:
Step No.Time(min)%A%B
104060
Flow Rate:
0.4 mL/min
Temperature:
28°C
Detection:
Tandem Mass Spec (MS-MS)
Detector Info:
3200 QTRAP
Sample Note:
SecurityGuard™ Guard Cartridge System extends column lifetime. - SecurityGuard Cartridges, PFP 4 x 2.0mm ID, 10/Pk Part No.: AJ0-8326 - Holder Part No.: KJ0-4282
Sample Preparation Details
Description:
Strata FL-PR Florisil (170 µm, 80 Å), 500 mg / 3 mL, Tubes, 50/Pk
Part Number:
Condition 1:
Methanol
Condition 2:
Methanol
Wash 1:
Methanol/Water (80:20)
Wash 2:
Methanol/Water (80:20)
Wash 3:
Methanol
Evaporation:
Evaporate to dryness
1
Condition
Methanol
2
Shake
Acetone/Water/Formic Acid (96:3.5:0.5)
1
Shake
Acetone/Water/Formic Acid (96:3.5:0.5)
2
Shake
Acetone/Water/Formic Acid (96:3.5:0.5)
1
Shake
Acetone/Water/Formic Acid (96:3.5:0.5)
2
Shake
Acetone/Water/Formic Acid (96:3.5:0.5)
3
Wash
Methanol

Order Items Used in This Application

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