Applications

Alfatoxins in Food by LC/MS/MS

Analytes

1

2

3

4

5

Compound Name Aflatoxin M1

Details

LC Conditions (App ID: 20016)

Column:

Luna 3 µm PFP(2) 100 Å, LC Column 50 x 2 mm, Ea

Brand Name:

Part No:

Elution Type:

Isocratic

Mobile Phase:

A: 5mM Ammonium Acetate + 0.1% Formic Acid

B: Methanol

Gradient Profile:

Step No.	Time(min)	%A	%B
1	0	40	60

Flow Rate:

0.4 mL/min

Temperature:

28°C

Detection:

Tandem Mass Spec (MS-MS)

Sample Preparation Details

Description: Strata FL-PR Florisil (170 µm, 80 Å), 500 mg / 3 mL, Tubes, 50/Pk
Part Number: 8B-S013-HBJ
Condition 1: 3 mL Methanol
Condition 2: 3 mL Methanol
Load: Load 1.50 mL Sample
Wash 1: 3 mL Methanol/Water (80:20)
Wash 2: 3 mL Methanol/Water (80:20)
Wash 3: 6 mL Methanol
Elution 1: 3 mL Acetone/Water/Formic Acid (96:3.5:0.5)
Elution 2: 3 mL Acetone/Water/Formic Acid (96:3.5:0.5)
Evaporation: Evaporate to dryness
Reconstitute: 1 mL Mobile Phase

Order Items Used in This Application

00B-4447-B0

Luna 3 µm PFP(2) 100 Å, LC Column 50 x 2 mm, Ea

$655.00 (Ea)

Qty:

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