Applications
Alfatoxins in Food by LC/MS/MS
20016
Separation Mode: Reversed Phase
Reversed Phase
PFP(2)
HPLC
Poisons
Food & Beverage
Biochemical Compound / Nutrient
Product / Trade Good
Environmental / Health
Therapeutic / Clinical

Applications

Alfatoxins in Food by LC/MS/MS
Analytes
1
2
3
4
5
Compound Name
Aflatoxin M1
Details
LC Conditions (App ID: 20016)
Column:
Brand Name:
Part No:
Elution Type:
Isocratic
Mobile Phase:
A: 5mM Ammonium Acetate + 0.1% Formic Acid
B: Methanol
Gradient Profile:
Step No.Time(min)%A%B
104060
Flow Rate:
0.4 mL/min
Temperature:
28°C
Detection:
Tandem Mass Spec (MS-MS)
Sample Preparation Details
Description:
Strata FL-PR Florisil (170 µm, 80 Å), 500 mg / 3 mL, Tubes, 50/Pk
Part Number:
Condition 1:
3 mL Methanol
Condition 2:
3 mL Methanol
Load:
Load 1.50 mL Sample
Wash 1:
3 mL Methanol/Water (80:20)
Wash 2:
3 mL Methanol/Water (80:20)
Wash 3:
6 mL Methanol
Elution 1:
3 mL Acetone/Water/Formic Acid (96:3.5:0.5)
Elution 2:
3 mL Acetone/Water/Formic Acid (96:3.5:0.5)
Evaporation:
Evaporate to dryness
Reconstitute:
1 mL Mobile Phase

Order Items Used in This Application

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