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Applications
Blank Plasma Samples Extracted from Phree Showing Endogenous Level of Vitamin D3 Using Kinetex C18
21870
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC

Blank Plasma Samples Extracted from Phree Showing Endogenous Level of Vitamin D3 Using Kinetex C18

Analytes

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Details
LC Conditions (App ID: 21870)
Column:
Brand Name:
Kinetex
Part No:
Phase Name:
C18
Sample Note:
Instrument: API 5000 (AB SCIEX) Ionization Source: APCI, Positive Polarity Ionization Source Parameters Curtain Gas: 25.0 psi Gas 1 (Neb Gas): 40.00 psi ihe: ON CAD (Collision Gas): 10.0 NC: 5.00 µA TEM: 360 °C Note: Gas 2 is not available in APCI source MRM Transitions and Mass Dependent Parameters: Compound Q1, Da Q3, Da Dwell, msec DP, V EP, V CE, V CXP, V Vit D2 (1) 395.3 209.3 45 69 10 25 10 Vit D2 (2) 395.3 269.2 45 69 10 25 10 IS (D3-3H2) 386.2 257.2 45 69 10 25 10 Vit D3 (1) 383.2 257.2 45 69 10 23 10 Vit D3 (2) 383.2 211.1 45 69 10 34 10
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