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Blank Plasma Samples Extracted from Phree Showing Endogenous Level of Vitamin D3 Using Kinetex C18
21870
Separation Mode: Reversed Phase
Reversed Phase
C18
HPLC
Blank Plasma Samples Extracted from Phree Showing Endogenous Level of Vitamin D3 Using Kinetex C18
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LC Conditions (App ID: 21870)
Column: |
Brand Name: Kinetex |
Part No: |
Phase Name: C18 |
Sample Note: Instrument: API 5000 (AB SCIEX)
Ionization Source: APCI, Positive Polarity
Ionization Source Parameters
Curtain Gas: 25.0 psi
Gas 1 (Neb Gas): 40.00 psi
ihe: ON
CAD (Collision Gas): 10.0
NC: 5.00 µA
TEM: 360 °C
Note: Gas 2 is not available in APCI source
MRM Transitions and Mass Dependent Parameters:
Compound Q1, Da Q3, Da Dwell, msec DP, V EP, V CE, V CXP, V
Vit D2 (1) 395.3 209.3 45 69 10 25 10
Vit D2 (2) 395.3 269.2 45 69 10 25 10
IS (D3-3H2) 386.2 257.2 45 69 10 25 10
Vit D3 (1) 383.2 257.2 45 69 10 23 10
Vit D3 (2) 383.2 211.1 45 69 10 34 10 |
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Phenomenex is a technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers worldwide