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Applications
Extraction of Procaine from Serum using Strata-X-C Microelution & a Kinetex Biphenyl HPLC Column
22983
Separation Mode: Reversed Phase
Reversed Phase
Biphenyl
HPLC
Anesthetics, Local

Extraction of Procaine from Serum using Strata-X-C Microelution & a Kinetex Biphenyl HPLC Column

Analytes
1Procaine
Details
LC Conditions (App ID: 22983)
Column:
Brand Name:
Kinetex
Part No:
Phase Name:
Biphenyl
Sample Note:
Strata X-C Elution Plate Protocol Condition 200 µL methanol Equilibrate 200uL water Load 750 µL diluted serum (375 µL plasma diluted 1:1 with 4% phosphoric acid in water) Wash1 200 µL 2% formic acid in water Wash 2 200 µL methanol Elute 25 µL 5% ammonium hydroxide in acetonitrile:methanol(60:40) Inject 1 µL Note: Spiked conc. of analyte was 1 ng/mL serum
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