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Applications
Vitamin D tablet assay (Up & Up) on a Kinetex 2.6 µm Biphenyl, 150 x 4.6
23494
Separation Mode: Reversed Phase
Reversed Phase
Biphenyl
HPLC
Vitamins

Vitamin D tablet assay (Up & Up) on a Kinetex 2.6 µm Biphenyl, 150 x 4.6

Analytes
1Triphenylene (I.S.)
2Vitamin D3
Details
LC Conditions (App ID: 23494)
Column:
Brand Name:
Kinetex
Part No:
Phase Name:
Biphenyl
Sample Note:
Sample preparation: small tablets (<400 mg) 1. Grind tablet into powder and transfer to a 2 mL dSPE tube (KS0-8916) 2. Spike in 20 µL of triphenylene internal standard (1 mg/mL) and standard additions (if applicable) 3. Add 800 µL of water 4. Sonicate until dissolved (approx. 5 min) 5. Add 800 µL of acetonitrile 6. Shake for 10 min using mechanical shaker 7. Centrifuge at 15000 rpm for 3 min 8. Aspirate 100 µL of supernatant and transfer to an autosampler vial for analysis Sample preparation: large tablets (>400 mg) 1. Grind tablet into powder and transfer to a 5 mL centrifuge tube 2. Spike in 20 µL of triphenylene internal standard (1 mg/mL) and standard additions (if applicable) 3. Add 2 mL of water 4. Sonicate until dissolved (approx. 5 min) 5. Add 400 mg of Quechers salt (AH0-9044) 6. Add 2 mL of acetonitrile 7. Shake for 10 min using mechanical shaker 8. Centrifuge at 6000 rpm for 3 min 9. Aspirate 100 µL of supernatant and transfer to an autosampler vial for analysis
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