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Dissolve 70 mg of Sildenafil Citrate in 1 mL of a solution of hydrogen peroxide and anhydrous formic acid (2:1). Allow to stand for at least 10 minutes to generate Sildenafil N-Oxide, then dilute w/mobile phase to 250 mL.
1. Sildenafil citrate
2. Sildenafil N-Oxide
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