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a. Measure out 1g of the cumin powder and place into a 50mL Centrifuge tube.
b. Add 10 mL of water followed by 15 mL of Acetonitrile.
c. Cap and shake well for 30 seconds
d. Place the tube into a centrifuge and spin at 3600RPM for 10 minutes.
e. Take 1.5mL of the supernatant and place into a separate vial as the Final Samples for Analysis