PhEur Atazanavir Sulfate - Impurity K; Reference solution D on Luna Omega 3u C18 150x4.6mm using Agilent 1260 Quaternary UHPLC system

Analytes

1Impurity K

Analyte not found

Details

Official Method: Ph.Eur.

LC Conditions (App ID: 27718)

Column:

Luna Omega 3 µm C18 100 Å, LC Column 150 x 4.6 mm, Ea

Brand Name:

Luna Omega

Part No:

00F-4784-E0

Phase Name:

C18

Elution Type:

Gradient

Mobile Phase:

A: mix 25 volumes of acetonitrile and 75 volumes of a freshly prepared 2.73 g/L solution of potassium dihydrogen phosphate previously adjusted to pH 3.5 with dilute phosphoric acid.

B: mix 25 volumes of a freshly prepared 2.73 g/L solution of potassium dihydrogen phosphate previously adjusted to pH 3.5 with dilute phosphoric acid, and 75 volumes of acetonitrile

Gradient Profile:

Step No.	Time(min)	%A	%B
1	0	95	5
2	5.6	95	5
3	8.6	0	100
4	14.6	0	100
5	16	95	5
6	23	95	5

Flow Rate:

1 mL/min

Temperature:

25°C

System:

Agilent Technologies 1260 Infinity Agilent Technologies 1260 Infinity

Detection:

LC/UV (DAD, PDA) @ 215.0000000000 nm (nanometers)

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PhEur Atazanavir Sulfate -Impurity K; Reference solution D on Luna 3u C18(2) 150x4.6mm using Agilent 1260 Quaternary UHPLC system

Luna 3 µm C18(2) 100 Å, LC Column 150 x 4.6 mm, Ea

Reversed Phase

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PhEur Atazanavir Sulfate - Impurity K; Reference solution D on Luna Omega 3u C18 150x4.6mm using Agilent 1260 Quaternary UHPLC system

Order Items Used in This Application

App ID 27718

Order Items Used in This Application